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Cell Isolation Core

The Cell Isolation Core provides isolated hepatocytes from human liver, technical support and experimental design for research investigators at KU Medical C and other KU campuses for a minimal cost. Hepatocytes can be used for a variety of studies that include drug metabolism, toxicology, enzyme activity assays and treatment of metabolic disease by cell transplantation. The lab is located in room 4052 of the state-of-the-art Hemenway Life Sciences Innovation Center.  The core is equipped with all of the necessary equipment and supplies for the isolation and culture of hepatocytes.  Some of the equipment includes:

  • 2 NuAire biological safety cabinets (model NU-425-400),
  • an Eppendorf refrigerated centrifuge (model 5810R),
  • an Eppendorf refrigerated microfuge (model 5424R),
  • a Cole-Parmer Masterflex L/S peristaltic pump (model 7523-90),
  • a NuAire water jacketed CO2 incubator (model NU-8700), and
  • a VanGuard inverted microscope with camera (model 1493Ini).

The Cell Isolation Core also provides snap frozen hepatocytes.  With the cooperation of the KU Liver Center and KU Hospital, the Cell Isolation Core can provide research investigators with a link to clinical investigators that will allow for greater understanding of liver disease and how to treat it.

The rates for the Cell Isolation Core services as of June 2022:

Cell type Perfusion Only Plated Cells
Hepatocytes, Human $50.00* $95.00**
Hepatocytes, Mouse $75.00 $110.00
Hepatocytes, Rat $90.00 $130.00
Non-parenchymal Cells Please contact the core for details. Please contact the core for details.

Available plate/flask/dish types include:  6-well plate, 12-well plate, 24-well plate, 48-well plate, 96-well plate, 25cm flask, 75cm flask, or 10cm dish.
1 Bottle (500 ml) of 'William's medium E' can also be provided at the cost of $50.00 per bottle.
*Price listed for per 1-5 million cells
**Price listed for per plate

JaeschkeHartmut Jaeschke Ph.D
Core Director
Ni PhotoHongmin Ni, Ph.D
Technical Director
School of Medicine

The University of Kansas Medical Center
Pharmacology, Toxicology & Therapeutics
Mailstop 1018
3901 Rainbow Boulevard
Kansas City, KS 66160