Cell Isolation Core
The Cell Isolation Core is a COBRE funded lab that provides isolated hepatocytes from human liver, technical support and experimental design for research investigators at KUMC and other KU campuses for a minimal cost. Hepatocytes can be used for a variety of studies that include drug metabolism, toxicology, enzyme activity assays and treatment of metabolic disease by cell transplantation. The lab is located in room 4052 of the state-of-the-art Hemenway Life Sciences Innovation Center. The core is equipped with all of the necessary equipment and supplies for the isolation and culture of hepatocytes. Some of the equipment includes: 2 NuAire biological safety cabinets (model NU-425-400), an Eppendorf refrigerated centrifuge (model 5810R), an Eppendorf refrigerated microfuge (model 5424R), a Cole-Parmer Masterflex L/S peristaltic pump (model 7523-90), a NuAire water jacketed CO2 incubator (model NU-8700) and a VanGuard inverted microscope with camera (model 1493Ini). The Cell Isolation Core also provides snap frozen hepatocytes and liver tissue as well as RNA, DNA and protein fractions. With the cooperation of the KU Liver Center and KU Hospital, the Cell Isolation Core can provide research investigators with a link to clinical investigators that will allow for greater understanding of liver disease and how to treat it.