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Lab Members

Current members of the X. Long Zheng Lab at the University of Kansas Medical Center, Kansas City, Kansas

Principal Investigator
X. Long Zheng, MD, PhD

Professor and Russell J. Eilers, MD, Endowed Chair
Department of Pathology & Laboratory Medicine

Education and Training
  • PhD, Mol. Cell Biology, University of Vienna, Vienna, Austria
  • MD, Medicine, Nanchang University, Nanchang, Jiangxi
  • Clinical Fellowship, Transfusion Medicine and Hemostasis, Washington University, St. Louis, Missouri
  • Residency, Pathology, Washington University , St. Louis , Missouri
  • Post Doctoral Fellowship, Hematology/Oncology, Washington University School of Medicine, St. Louis, Missouri
  • Post Doctoral Fellowship, Biological Science, Wichita State University, Wichita, Kansas

Wenjing (Wendy) Cao, MD, PhD

Research Assistant Professor
Assistant director of the Special Coagulation lab
Department of Pathology and Laboratory Medicine
University of Kansas Medical Center


MD/PhD, Tongji Medical College, Huazhong University of Science and Technology, China
Postdoctoral Fellow, The Children’s Hospital of Philadelphia, University of Pennsylvania 

Research interests:

My research focuses on molecular components and mechanisms of blood coagulation and associated disorders, in particular thrombotic thrombocytopenia purpura (TTP) and Hemophilia A. My current projects involve in 1) Cofactors dependent regulation of VWF function; 2) FVIII biology and its interaction with VWF; 3) Novel biomarkers that contribute to the pathogenesis, diagnosis, and therapeutic targets of TTP.  

  • Cao W, Krishnaswamy S, Camire RM, Lenting PJ, Zheng XL. Factor VIII accelerates proteolytic cleavage of von Willebrand factor by ADAMTS13. Proc Natl Acad Sci U S A, 2008 May 27; 105(21): 7416-7421
  • Cao WJ, Niiya M, Zheng XW, Shang DZ, Zheng XL. Inflammatory cytokines inhibit ADAMTS13 synthesis in hepatic stellate cells and endothelial cells. J Thromb Haemost. 2008 Jul; 6(7): 1233-5
  • Niiya M, Endo M, Shang D, Zoltick PW, Muvarak NE, Cao W, Jin SY, Skipwith CG, Motto DG, Flake AW, Zheng XL. Correction of ADAMTS13 Deficiency by In Utero Gene Transfer of Lentiviral Vector encoding ADAMTS13 Genes. Mol Ther. 2009 Jan; 17(1): 34-41
  • Laje P, Shang D, Cao W, Endo M, Niiya M, Zoltick PW, Alan W. Flake AW and Zheng XL. Correction of murine Adamts13 deficiency by hematopoietic progenitor cell-mediated gene therapy. Blood. 2009 Mar 5;113 (10):2172-2180
  • Raife TJ, Cao W, Atkinson BS, Bedell B, Montgomery RR, Lentz SR, Johnson GF, and Zheng XL. Leukocyte proteases cleave von Willebrand factor predominantly at or near the ADAMTS13 cleavage site. Blood. 2009 Aug 20;114(8):1666-1674
  • Zhou W, Chen KH, Cao W, Zeng J, Liao H, Zhao L, Guo X. Mutation of the protein kinase A phosphorylation site influences the anti-proliferative activity of mitofusin 2. Atherosclerosis, 2010 Jul;211(1):216-223
  • Skipwith CG, Cao W, Zheng XL. FVIII and platelets synergistically accelerate cleavage of von Willebrand factor by ADAMTS13 under fluid shear stress. J Bio Chem, 2010 Sep 10; 285(37):28596-603
  • Cao W, Sabatino DE, Altynova E, Lange AM, Casina VC, Camire RM, Zheng XL. Light chain of factor VIII is sufficient for accelerating cleavage of von Willebrand factor by ADAMTS13 metalloprotease. J Biol Chem, 2012 Sep 21;287(39):32459-66
  • Wang Q, Dong B, Firrman J, Roberts S, Moore AR, Cao W, Diao Y, Kapranov P, Xu R, Xiao W. Efficient production of dual recombinant adeno-associated viral vectors for factor VIII delivery. Hum Gene Ther Methods. 2014 Aug;25(4):261-268
  • Zander CB, Cao W, Zheng XL. ADAMTS13 and von Willebrand factor interactions. Curr Opin Hematol, 2015 Sep;22(5):452-459
  • Cao W, Pham HP, Williams LA, R, McDaniel J, Lorenz RG, Marisa B. Marques MB, and Zheng XL. Human neutrophil peptides and complement factor bb in pathogenesis of acquired thrombotic thrombocytopenic purpura. Haematologica, 2016 Nov;101(11):1319-1326
  • Williams LA, Drwiega J, Cao W, Pham HP, Zheng XL. Acquired autoimmune thrombotic thrombocytopenic purpura in a case of severe hemophilia A. Haemophilia, 2016 Nov;22(6): e565-e567
  • Cao W and Zheng XL. Conformational quiescence of ADAMTS-13 prevents proteolytic promiscuity: comment. J. Thromb. Haemost, 2017 Mar;15(3):586-589
  • Kumar M*, Cao W*, McDaniel JK, Pham HP, Raju D, Nawalinski K, Frangos S, Kung D, Zager E, Kasner SE, Levine JM, Zheng XL. Plasma ADAMTS13 activity and von Willebrand factor antigen and activity in patients with subarachnoid haemorrhage. Thromb Haemost 2017 Apr 3;117(4):691-699 (*Co-1st author)
  • Russell RT, McDaniel JK, Cao W, Shroyer M, Wagener BM, Zheng XL, Pittet JF. Low plasma ADAMTS13 activity is associated with coagulopathy, endothelial cell damage and mortality after severe paedeatric trauma. Thromb Haemost, 2018 Apr;118(4):676-687
  • Abdelgawwad MS, Cao W, Zheng L, Kocher NK, Williams LA, and Zheng XL. Transfusion of platelets loaded with recombinant ADAMTS13 is efficacious for inhibiting arterial thrombosis in mice and in human. Arterioscler Thromb Vasc Biol, 2018 Nov;38(11):2731-2743
  • Staley EM*, Cao W*, Pham HP, Kim CH, Kocher NK, Zheng L, Gangaraju R, Lorenz RG, Williams LA, Marques MB, and Zheng XL. Clinical factors and biomarkers predicting outcome in patients with immune-mediated thrombotic thrombocytopenic purpura. Haematologica, 2019 Jan;104(1):166-175 (*Co-1st author)
  • Kumar MA*, Cao W*, Pham HP, Raju D, Nawalinski K, Maloney Wilensky E, Schuster JM, Zheng XL. Relative Deficiency of Plasma ADAMTS13 Activity and Elevation of Human Neutrophil Peptides in Patients with Traumatic Brain Injury. J Neurotrauma, 2019 Jan 15;36(2):222-229 (*Co-1st author)
  • Tang N, Yu F, Cao W, and Wang X. Significantly prolonged PT and APTT could indicate a wide spectrum of clinical manifestations in three patients with plasma cell disorders. Int J Lab Hematol, 2019 Feb;41(1): e23-e26.
  • McDaniel JK, Abdelgawwad MS, Hargett A, Renfrow MB, Bdeir K, Cao W, Cines DB, Zheng XL. Human neutrophil peptide-1 inhibits thrombus formation under arterial flow via its terminal free cysteine thiols. J Thromb Haemost. 2019 Apr;17(4):596-606.
  • Zheng L, Zhang D, Cao W, Song WC, Zheng XL. Synergistic effects of ADAMTS13 deficiency and complement activation in pathogenesis of thrombotic microangiopathy. Blood. 2019 Sep 26;134(13):1095-1105.
  • Cao W, Abdelgawwad MS, Li J, Zheng XL. Apolipoprotein B100/Low-Density Lipoprotein Regulates Proteolysis and Functions of von Willebrand Factor under Arterial Shear. Thromb Haemost, 2019 Dec;119(12):1933-1946. doi: 10.1055/s-0039-1696713. Epub 2019 Sep 7.
  • Zheng L, Abdelgawwad MS, Zhang D, Xu L, Wei S, Cao W, and Zheng XL. Histone-Induced Thrombotic Thrombocytopenic Purpura in Adamts13-/- Zebrafish Depends On Von Willebrand Factor. Haematologica 2020 Apr; 105(4):1107-1119
  • Sui J, Cao W, Halkidis K, Abdelgawwad MS Kocher NK, Guillory BK, Williams LA, Gangaraju R, Marques MB, X. Long Zheng. Longitudinal Assessments of ADAMTS13 Biomarkers Predict Recurrence of Immune Thrombotic Thrombocytopenic Purpura. Blood Adv. 2019 Dec 23;3(24):4177-4186
  • Cao W*, Cao W*, Zhang W, Zheng XL, Zhang XF. Factor VIII binding affects the mechanical unraveling of the A2 domain of von Willebrand factor. J Thromb Haemost. 2020 Sep;18(9):2169-2176 (*Co-first author)
  • Fei Y, Tang N, Liu H, Cao W. Coagulation Dysfunction: A hallmark in COVID-19. Arch Pathol Lab Med. 2020 Oct 1;144(10):1223-1229
  • Cao W, Dong B, Horling F, Firrman JA, Lengler J, Klugmann M, de la Rosa M, Wu W, Wang Q, Wei H, Moore AR, Roberts SA, Booth CJ, Hoellriegl W, Li D, Konkle B, Miao C, Reipert BM, Scheiflinger F, Hanspeter Rottensteiner H, Xiao W. Minimal essential human factor VIII alterations enhance secretion and gene therapy efficiency. Mol Ther Methods Clin Dev. 2020 Dec,19(4):486-495
  • Sui J, Lu R, Halkidis K, Kocher NK, Cao W, Marques MB, Zheng XL. Plasma levels of S100A8/A9, histone/DNA complexes, and cell-free DNA predict adverse outcomes of immune thrombotic thrombocytopenic purpura. J Thromb Haemost. 2021 Feb;19(2):370-379
  • Basu MK, Massicano F, Yu L, Halkidis K, Pillai V, Cao W, Zheng L, Zheng XL. Exome Sequencing Identifies Abnormalities in Glycosylation and ANKRD36C in Patients with Immune-Mediated Thrombotic Thrombocytopenic Purpura. Thromb Haemost. 2021 Apr;121(4):506-517
Konstantine Halkidis, MD, PhD

Assistant Professor
Physician Scientist
Division of Hematologic Malignancies and Cellular Therapeutics
Department of Internal Medicine

Education and Training:

MD/PhD: Thomas Jefferson University
Fellowship: Hematology, University of Alabama, Birmingham
Residency: Internal Medicine, Drexel University College of Medicine
BS: Biochemistry, Temple University

Research interests:

Mechanistic biochemistry

Szumam Liu, PhD

Postdoctoral Fellow


Combined M.A. and Ph.D. Program: Department of Biomedical Sciences and Engineering, National Central University (Taiwan)

Postdoctoral Fellowship: Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham

Research interests:

My research work focus is on the role of anti-ADAMTS13 autoantibodies in thrombotic thrombocytopenic purpura (TTP) patients.

  • Su H, Jiang M, Senevirathne Chamara, Aluri Srinivas, Zhang T, Xavier-Ferrucio Juliana, Tran NT, Liu SM, Guo H, Jin S, Zhu Y, Zhao Q, Chen Y, Cable LouAnn, Shen Y, Liu J, Qu CK, Klug Christopher A., Bhatia Ravi, Chen Y, Nimer Stephen D., Zheng Y, Jin J, Deng H, Krause Diane S., Xiang J, Verma Amit, Luo M, and Zhao X. Methylation of Dual Specificity Phosphatase 4 Controls Cell Differentiation. Cell Report, 2021, 27:36(4):109421

  • Thi Nguyen MH, Lin CH, Liu SM, Miyashita A, Ihn H, Lin H, Ng CH, Tsai JC, Chen MH, Tsai MS, Lin IY, Liu SC, Li LY, Satoshi Fukushima, Lu Jean and Ma N. miR-524-5p reduces the progression of the BRAF inhibitor-resistant melanoma, Neoplasia, 2020, 31;22(12):789-799

  • Su H, Sun CW, Liu SM, He X, Hu H, Pawlik KM, Townes TM, Han X, Klug CA, Henary M, Chen Y, Li L, Zheng YG, Zhao X, Defining the epigenetic status of blood cells using a cyanine-based fluorescent probe for PRMT1, Blood adv, 2018, 13;2(21):2829-2836.

  • Liu SM, Lin CH, Lu J, Lin IY, Tsai MS, Chen MH, Ma N. miR-596 modulates melanoma growth by regulating cell survival and death, J Invest Dermatol, 2017, 138(4):911-921.

  • Hsu CL, Chung FH, Chen CH, Hsu ZT, Liu SM, Chung DS, Hsu YF, Chen CL, Ma N & Lee HC. Genotypes of cancer stem cells characterized by epithelial-to mesenchymal transition and proliferation related functions, Scientific Reports, 2016, 6:32523.

  • Yen CI, Liu SM, Lo WS, Wu JW, Liu YH, Chein RJ, Yang R, Wu KC, Hwu JR, Ma N, Shieh FK. Cytotoxicity of Postmodified Zeolitic Imidazolate Framework-90 (ZIF-90) Nanocrystals: Correlation between Functionality and Toxicity, Chemistry, 2016, 24;22(9):2925-9.

  • Lo W-S, Liu SM, Wang S-C, Lin H-P, Ma N, Huang H-Yand Shieh F-K, A green and facile approach to obtain 100 nm zeolitic imidazolate framework-90 (ZIF-90) particles via leveraging viscosity effects, RSC Advances, 2014, 4:52883-86.

  • Liu SM, Lu J, Lee, HC, Chung FH, and Ma N, miR-524-5p suppresses growth of oncogenic BRAF melanoma by targeting BRAF and ERK2, Oncotarget, 2014, 5(19):9444-59.

Chan Meng, MD

Research Associate

Noritaka Yada, MD, PhD

Postdoctoral Fellow 
Department of Pathology and Laboratory Medicine
University of Kansas Medical Center

Education, Professional Training & Employment:

MD: University of Tottori, School of Medicine, Tottori, Japan (2005)
Residency: Tottori Prefectural Central Hospital, Ezu, Tottori, Japan (2007)
Clinical Fellow: Deptartment of Emergency and Critical Care, Medicine in Yodogawa Christian Hospital (2007-2009); Dept. of Emergency and Critical Care, Medicine in Nara Medical University (2009-2010); Dept. of Emergency and Critical Care Medicine in Yodogawa Christian Hospital (2010-2015)
Assistant Professor:  Department of General Medicine, Nara Medical University, Kashihara, Nara, Japan (2015-2020)
PhD: Nara Medical University, Kashihara, Nara, Japan (2020)

Research projects:

Neutrophil extracellular traps (NETs) and the formation of microvascular thrombosis in immune thrombotic thrombocytopenic purpura  

  • Plasma level of von Willebrand factor propeptide at diagnosis: a marker of subsequent renal dysfunction in autoimmune rheumatic diseases. Noritaka Yada, Kiyomi Yoshimoto, Hiromasa Kawashima, Ryo Yoneima, Nobushiro Nishimura, Yoshiaki Tai, Emiko Tsushima, Makiko Miyamoto,Shiro Ono, Masanori Matsumoto, Takashi Fujimoto, and Kenji Nishio. Clinical and Applied Thrombosis/Hemostasis. 2020; 26:1076029620938874. Published online 2020 Jul24. doi: 10.1177/1076029620938874.

  • Early differential diagnosis between thrombotic microangiopathy (TMA) and disseminated intravascular coagulation (DIC). Noritaka yada, Kenji Nishio. Jpn J Thromb Hemost. 2020; 31: 7-16.

  • Importance of Laboratory Detection of Macro-Aspartate Aminotransferase. Shiro Ono, Chikara Kurata, Nobushiro Nishimura, Hiromasa Kawashima, Ryo Yoneima, Yoshiaki Tai, Emiko Tatsumi, Makiko Miyamoto, Noritaka Yada, Kiyomi Yoshimoto, Kenji Nishio. International Journal of General Medicine. 2019; 12: 433-436.

  • Functional regulation of von Willebrand factor ameliorates acute ischemia-reperfusion kidney injury in mice. Shiro ono, Hideto Matsui, Masashi Noda, Shogo Kasuda, Noritaka Yada, Kiyomi Yoshimoto, Masashi Akiyama, Toshiyuki Miyata,Mitsuhiko Sugimoto, Kenji Nishio. Scientific Reports. 2019; 9: 14453. doi: 10.1038/s41598-019-51013-2.

  • Interventional radiology versus operative management for splenic injuries: a study protocol for a systematic review and meta-analysis. Masahiro Kashiura, Noritaka Yada, Kazuma Yamakawa. BMJ Open. 2019; 9: e028172. doi:10.1136/bmjopen-2018-028172.

  • STEC: O111-HUS complicated by acute encephalopathy in a young girl was successfully treated with a set of hemodiaļ¬ltration, steroid pulse, and soluble thrombomodulin under plasma exchange. Noritaka Yada, Masayuki Fujioka, Charles L. Bennett, Kazuya Inoki, Toyokazu Miki, Akihiko Watanabe, Toshiko Yoshida, Masaki Hayakaw, Masanori Matsumoto, Yoshihiro Fujimura. Clinical Case Report. 2015; 3: 208-212.

  • Therapeutic effect of recombinant thrombomodulin on the hemostatic and inflammatory response in patients with septic DIC. Noritaka Yada, Kenji Nishio, Tadahiko Seki, Hidetada Fukushima, Yasuyuki Urizono, Michiaki Hata, Kazuo Okuchi. Journal of Japanese Association for Acute Medicine. 2011; 22: 749-757. 

  • Out-of-hospital cardiac arrest caused by acute intoxication. Hidetada Fukushima, Tomoo watanabe, Hideaki Asai, Noritaka Yada, Shingo Ito, Tadahiko Seki, Toru Ueyama, Yasuyuki Urizono, Kenji Nishio, Kazuo Okuchi. Chudoku Kenkyu. 2010; 23: 41-46.

Quan Zhang, MD

Visiting Researcher
Department of Pathology and Laboratory Medicine
University of Kansas Medical Center

Education, Professional Training & Employment:

MD: School of Medicine, Hunan University of Chinese Medicine, Changsha, China

Residency: Department of Critical Care Medicine, The First Hospital of Changsha, China

MS: The Second Xiangya Hospital, Central South University, Changsha, China

PhD (Candidate): Respiratory Medicine, The Second Xiangya Hospital, Central South University, Changsha, China

Research interests:

NETs formation and ADAMTS13-von Willebrand factor axis promote thrombosis in COVID-19 and vascular diseases.

  • Zhang Q, Peng F, Li MZ, et al. Elevated Risk of Venous Thromboembolism in People Living with HIV. Viruses. 2022 Mar 12; 14 (3): 590.
  • Zhang Q, Peng F, Lei Si, et al. Next-Generation Sequencing in Diagnosis of Atypical Aspiration Pneumonia. Front Public Health. 2022 Jan 4; 9: 771154.
  • Peng F, Lei S, Zhang Q, et al. Triglyceride/High-Density Lipoprotein Cholesterol Ratio is Associated with the Mortality of COVID-19: A Retrospective Study in China. Int J Gen Med. 2022 Jan 31; 15: 985-996.
  • Zhang Q, Huang K, Lv JL, et al. Case Report: Human Umbilical Cord Mesenchymal Stem Cells as a Therapeutic Intervention for a Critically Ill COVID-19 Patient. Front Med (Lausanne), 2021 Jul 8; 8: 691329.
  • Peng F, Lei S, Zhang Q, et al. Smoking Is Correlated with the Prognosis of Coronavirus Disease 2019 (COVID-19) Patients: An Observational Study. Front Physiol. 2021 Mar 3; 12: 634842. 
  • Wang GY, Zhang Q, Wu CF, et al. Clinical Characteristics of Adult Fevered COVID-19 Patients and Predictors for Developing Severe Events. Front Med (Lausanne), 2020 Jul 3; 7: 324.
  • Yao YH, Luo Y, Zhang Q, et al. The effect of a year of highly active antiretroviral therapy on immune reconstruction and cytokines in HIV/AIDS patients. AIDS Res Hum Retroviruses. 2013 Apr; 29 (4): 691-7.

Liang Zheng, PhD

Research Assistant Professor


PhD:  Hokkaido University, Japan
Postdoctoral Fellowship:  University of Illinois at Chicago
Postdoctoral Fellowship:  University of Alabama at Birmingham

Research interests:

My research interests involve elucidation of the disease mechanism of Thrombotic Microangiopathy (TMA), a group of rare yet life-threatening thrombotic disorders, and development of new therapeutics in the animal models of TMA. Using the zebrafish and mouse models, my current work focuses on the identification and characterization of genetic factors that modulate the pathogenesis of TMA, and elucidation of the role of environmental factors that may act as disease modifiers.

  • Histone-induced thrombotic thrombocytopenic purpura in adamts13 -/-zebrafish depends on von Willebrand factor. Zheng L, Abdelgawwad MS, Zhang D, Xu L, Wei S, Cao W, Zheng XL. Haematologica. 2020 Apr;105(4):1107-1119. doi: 10.3324/haematol.2019.237396. Epub 2019 Nov 21.PMID: 31753928 

  • Synergistic effects of ADAMTS13 deficiency and complement activation in pathogenesis of thrombotic microangiopathy. Zheng L, Zhang D, Cao W, Song WC, Zheng XL.Blood. 2019 Sep 26;134(13):1095-1105. doi: 10.1182/blood.2019001040. Epub 2019 Aug 13.PMID: 31409673  

  • Exome Sequencing Identifies Abnormalities in Glycosylation and ANKRD36C in Patients with Immune-Mediated Thrombotic Thrombocytopenic Purpura. Basu MK, Massicano F, Yu L, Halkidis K, Pillai V, Cao W, Zheng L, Zheng XL.Thromb Haemost. 2021 Apr;121(4):506-517. doi: 10.1055/s-0040-1719030. Epub 2020 Nov 12.PMID: 33184803

  • Transfusion of Platelets Loaded With Recombinant ADAMTS13 (A Disintegrin and Metalloprotease With Thrombospondin Type 1 Repeats-13) Is Efficacious for Inhibiting Arterial Thrombosis Associated With Thrombotic Thrombocytopenic Purpura. Abdelgawwad MS, Cao W, Zheng L, Kocher NK, Williams LA, Zheng XL.Arterioscler Thromb Vasc Biol. 2018 Nov;38(11):2731-2743. doi: 10.1161/ATVBAHA.118.311407.PMID: 30354235

  • Therapeutic efficacy of the platelet glycoprotein Ib antagonist anfibatide in murine models of thrombotic thrombocytopenic purpura. Zheng L, Mao Y, Abdelgawwad MS, Kocher NK, Li M, Dai X, Li B, Zheng XL.Blood Adv. 2016 Nov 29;1(1):75-83. doi: 10.1182/bloodadvances.2016000711.PMID: 28480350

  • Effects of ibuprofen, diclofenac and paracetamol on hatch and motor behavior in developing zebrafish (Danio rerio). Xia L, Zheng L, Zhou JL.Chemosphere. 2017 Sep;182:416-425. doi: 10.1016/j.chemosphere.2017.05.054. Epub 2017 May 10.PMID: 28511137

  • Transcriptional and morphological effects of tamoxifen on the early development of zebrafish (Danio rerio). Xia L, Zheng L, Zhou JL.J Appl Toxicol. 2016 Jun;36(6):853-62. doi: 10.1002/jat.3257. Epub 2015 Nov 20.PMID: 26584595

  • Quantitative toxicoproteomic analysis of zebrafish embryos exposed to a retinoid X receptor antagonist UVI3003. Zheng L, Yu J, Shi H, Xia L, Xin Q, Zhang Q, Zhao H, Luo J, Jin W, Li D, Zhou J.J Appl Toxicol. 2015 Sep;35(9):1049-57. doi: 10.1002/jat.3099. Epub 2015 Jan 11.PMID: 25581642

  •  A representative retinoid X receptor antagonist UVI3003 induced teratogenesis in zebrafish embryos. Zheng L, Xu T, Li D, Zhou J.J Appl Toxicol. 2015 Mar;35(3):280-6. doi: 10.1002/jat.3051. Epub 2014 Sep 3.PMID: 25186191

Antonia Bignotti, BS

Research Assistant

Aria Trask, BA

Graduate Student

KU School of Medicine

University of Kansas Medical Center
Department of Pathology & Laboratory Medicine
Mail Stop 3045
3901 Rainbow Boulevard
Kansas City, KS 66160
Phone: 913-588-7070
Fax: 913-588-7073