Brent Sear

Professional Background

I am interested in studying the origin and activity of the enzyme β-Glucuronidase (β-Gluc) in bladder cancer patients. β-Gluc is most commonly secreted by renal tubular epithelial cells. It is a member of the glycosidase family of enzymes that breaks down complex carbohydrates. The function of this enzyme is to detoxify/inactivate several substances, including but not limited to hormones, neurotransmitters, and toxins. β-Gluc can also reactivate carcinogenic compounds that have been conjugated by the liver for urinary elimination. Research has shown that diseases which cause tissue damage to the urinary tract, such as bladder cancer, can cause an increase in urinary β-Gluc activity.

With my research, I aim to develop and validate a specific bioanalytical method to quantify β-Gluc activity in urine across mouse and human species, as well as identify the source or origin of β-Gluc production and its release into urine. These two aims will allow me to determine whether β-Gluc activity in urine is increased in bladder cancer patients.

• Bachelor of Science in Cell Biology, University of Kansas
• Bachelor of Science in Pharmaceutical Science, University of Kansas
• Doctorate of Pharmacy, University of Kansas