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Sample Preparation Standards

We encourage all prospective users of this facility to consult with us before submitting any sample for MS analysis (please see Policies).

All samples must be accompanied by a sample submission form with detailed information of the sample submitted. When the samples are from SDS-PAGE, include a picture of the Coomassie stained gel.

Samples in solution must be clearly labeled and should be submitted in 0. 5 ml or smaller Eppendorf-type tubes. Although purity is not a requirement for LC/MS and ESI, samples should be solvent-free and if possible in water. Contaminants present in the sample could suppress the signal of the protein of interest or they can make the spectra difficult to interpret.

Protein Solutions for Gel-Free Analysis
Protein solutions should be frozen at -80°C as soon as possible and shipped to us on dry ice.
Note about proteins and peptides: Proteins and peptides in solution should not be stored in glass vials. It is our experience that proteins will adsorb irreversibly onto the glass. Samples in solution should be stored in 0.25 or 0.5 ml Eppendorf-type centrifuge tubes.

Sample size:

The following recommendations are general guidelines. Our current instrumentation is extremely sensitive and most likely will be able to perform a good measurement with less material than indicated.

Soluble samples: For gel-free protein characterization, we find that 5-10 µg of total protein will provide enough sample for several good LC-MS analyses. This will allow us to confirm identifications and use exclusion lists to identify some of the less abundant proteins in the sample. The concentration (mg/ml) of the sample needed depends on the molecular mass of the protein/peptide.  Table 1 is given for reference purposes.

Table 1: Mass dependence of protein concentration for mass spectrometric analysis of soluble protein*

Mass (Da) Concentration required in mg/ml
(20-50 µM)





























* This is a general guideline; current instrumentation is more sensitive.

PAGE bands: Coomassie-stained gels are preferred. The amount of sample required to ensure an adequate protein identification attempt is approximately 50-150 fmol (approximately 3-15 ng of a 100 Kd protein).  This represents the lower limit of detection in the average colloidal Coomassie blue stained mini-gel. Users are encouraged to scale up sample preparation to a level that can be detected with Coomassie-stained gels.  It is our experience that spending some time doing method development will result in quicker, less ambiguous protein identification. If not possible, SYPRO Ruby-stained gels can also be submitted. Silver stained gels, even MS-compatible silver stains, are strongly discouraged. 

Radioactive Sample Permissible Levels. None.

Please review the following document: keratin-free environment

In-Gel digests and Protein ID by MS. The facility offers automatic sample processing of 1-D and 2-D gels, in preparation for analysis by MS. Gel bands from 1D gel electrophoresis or gel spots obtained from 2D gel approaches should be cut with the following considerations in mind:

  1. Beware of contaminating proteins in all phases of sample handling. Dust particles contain high levels of proteinaceous material, especially skin and hair proteins of which the members of the keratin class are the most abundant. As early as possible, in the sample preparation, step "anti-dust" measures should be introduced. These measures include thoroughly washing all glassware and plasticware, having dedicated "mass spectrometry" solutions that have been filtered, always wearing gloves, lab coats, and covering the gel through all phases of staining and destaining. When we engage in manual gel cutting, we perform this step in a laminar flow hood to reduce the possibility of dust contamination.
  2. Try to keep the volume of gel matrix material to a minimum. Excess amounts of polyacrylamide will reduce the yield of identifiable peptides from a band or spot. Try to cut away as much unstained gel material as possible.
  3. Keeping the protein contained within the gel slice/band slightly acidified reduces the activity of the alkaline proteases. We typically store our gel bands/spots at 4°C in 1% acetic acid.
  4. Do not freeze the gel bands/spots since thawing gel bands that have previously been frozen results in leakage of intact proteins from the gel matrix.

The excised gel material should be placed in individual 0.25 or 0.5 ml microcentrifuge tubes or a 96 well plate. We could provide you with 96 well plates that are custom built for our digestion robot, which will reduce sample handling and reduce potential sources of contamination.

Detergents and Mass Spectrometry1

Please talk to us about any detergents that are used in your sample preparation procedure, even if they are far upstream in your sample handling protocol.
Unfortunately, most detergents are not compatible with downstream mass spectrometry analysis by ESI mass spectrometry. Dilution, washing, and detergent removal columns cannot remove enough detergent for successful analysis of your sample or to prevent significant contamination of the mass spectrometer. Detergent contamination of a mass spectrometer is very costly (ruined columns/tubing) and very time-consuming to the laboratory. If your samples are very complex, it might be possible to purify them by running them through an SDS-PAGE gel.

In-solution Digestion

  • Compatible Detergents
    • 0.05%-1% SDS
    • 0.05%-0.5% CHAPS (although not recommended)
  • Incompatible Detergents
    • Nonidet P-40 (which can no longer be purchased; Sigma is substituting CA- Igepal 630)
    • Triton® X-100 (or any derivative)
    • Igepal/PEG (any derivative)
    • Brij®-35 (or any derivative)
    • Tween®-20 OTG
    • CHAPSO
    • Type NP40/NP40 alternative

In-gel Digestion

  • Compatible Detergents
    • SDS (up to 2%)
    • CHAPS (up to 4%) (although not recommended)
    • Nonidet P-40 (up to 1%); which can no longer be purchased; Sigma is substituting CA-Igepal 630 (not compatible)
  • Incompatible Detergents
    • Triton® X-100 (or any derivative)
    • Tween®-20
    • Igepal/PEG (any derivative)
    • Brij®-35 (or any derivative)
    • OTG
    • CHAPSO
    • Type NP40/NP40 alternative

Some detergents can be separated from the sample by standard SDS-PAGE or TCA - cold acetone precipitation. However, Triton-X and Tween-20 cannot be used under any circumstances. These cannot be removed from your sample using dilution, washing, detergent spin columns, or SDS-PAGE.

Other Mass Spec Friendly Detergents

    • 1) N-octyl-β-glucopyranoside
    • 2) PPS Silent Surfactant (acid-cleavable detergent)
    • 3) Protea Biosciences (anionic, zwitterionic, or cationic acid labile detergents)
    • 4) Big CHAP deoxy (merck) h
    • 5) ASB series (EMD chemicals)
    • 6) sodium deoxycholate

ESI-MS and ESI-MS/MS.  ESI-MS analysis will be performed on a LTQ-FT mass spectrometer.  MS/MS will be done automatically under software control. In general, duplicate MS/MS will be acquired on the three to six most intense ions detected on the previous full-MS scan; ion exclusion will be turned on for 90 seconds. Background ions (i.e., calibration standards, tryptic peptides, etc.) will be excluded. Mass accuracy can be as good as 2 ppm, depending on detector settings.

Mass Spectrometry/Proteomics Core Laboratory

University of Kansas Medical Center
Mass Spectrometry/Proteomics Core Laboratory
1058 Hemenway
3901 Rainbow Boulevard
Kansas City, Kansas 66160