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Mass Spectrometry/Proteomics Core Laboratory Equipment

  • Orbitrap Fusion Lumos (ThermoFisher) equipped with a nano electrospray source and connected to on-line uHPLC (EasynLC 1200)
  • Triple Quadrupole (Quantum AM ThermoFisher ) equipped with a nano electrospray source
  • Automatic Gel Slicer with gel documentation and DIGE capabilities (ProPicII, DIGILAB Genomic Solutions)
  • Automatic Digestor (ProGest, DIGILAB Genomic Solutions)
  • Off-line fractionation: The Mechano

Search engines/computer software

  • Database search servers: SEQUEST Cluster, Proteome Discoverer (version 2.2) and MaxQuant. The Sequest cluster is running on a dedicated 8 node double processor server. The Proteome Discoverer and MaxQuant are running on a 32 processor workstation.
  • PEAKS (from Bioinformatics solutions): This is a new software tool for protein identification, quantization, and de novo peptide sequencing.
  • HDExaminer (from Sierra Analytics): This program is now our preferred software for the analysis of protein dynamics by hydrogen/deuterium exchange mass spectrometry.

Custom-built tools

  • SAIDE: This is a cooling chamber for hydrogen/deuterium exchange mass spectrometry experiments that interfaces with the HPLC and MS. Our design was built by Mecour.
  • AIDE: This is a fully automatic interface for hydrogen/deuterium exchange mass spectrometry. This new interface has two working modes: quench flow for sub-second to 30 second time scale and normal for reactions times above 30 sec.
  • Pressure cells: We use pressure cells to pour a variety of columns used in nano HPLC. Our pressure cells were built by ArtBel (e-mail: esloquehayrally@gmail.com).
Mass Spectrometry/Proteomics Core Laboratory

University of Kansas Medical Center
Mass Spectrometry/Proteomics Core Laboratory
1058 Hemenway
3901 Rainbow Boulevard
Kansas City, Kansas 66160