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Services and Equipment

Confocal


A1R2037a inverted Eclipse TiE with A1R confocal

Nikon Eclipse TiE inverted motorized microscope with perfect focus is attached to an A1R-SHR confocal, with an A1-DU4 detector and an A1-DUT Transmitted detector.  The system has a four-line LU4 laser launch with 404nm, 488nm, 561nm, and 640nm solid state (OPSL) lasers. It is equipped with both high-speed resonant and high-resolution Galvano confocal scanning modes. This system also has TIRF microscopy capabilities with a 405/488/561/638 quad cube and Quad, 525/50,60/50/ and 700/75 emission filters with NiDAQ fast triggering. TIRF allows for the selective excitation of fluorophores within ~100nm of the coverslip and on this system emissions are imaged with an iXon Ultra 897 Andor EMCCD camera, which has high quantum efficiency, extremely low noise (<e- with EM gain), and a fast readout of up to 17MHz. This system also has a stage top incubation system, Chamlide TC, for live-cell imaging with humidity and temperature controls, and it is currently hooked up to a 5% CO2 compressed air tank (tank located in 2047). More information and scheduling at www.Bookkit.org


Fluorescent compound microscopes


90i2047 90i upright fluorescent microscope with motorized stage

Brightfield and Fluorescence

Objectives: 4X/10X/20X

DIC M N2Plan Fluor 40x , DIC M/N2Plan Apo 60x

Epi cubes

UV-2E/C Excitation 360/40nm, Dichroic 400nm, Emission 460/50 nm

CY GFP Ex436/20, Di455, Em480/40

HQ:F Ex480/40, Di505, Em535/50

TXRED-4040B-NTE Semrock Brightline Ex562/40, Di593, Em624/40

HQ:Cy5 Ex620/60, Di660, Em700/75

More information and scheduling at www.Bookkit.org


ti20002047 TE2000E inverted motorized fluorescent microscope

Brightfield and Fluorescence

Objectives

Plan Apo 4x /10X

Plan Apo/Fluor ELWD 20x

Plan Fluor ELWD 40x

Plan Apo 60x 

Epi cubes 

V-2A Nikon Excitation 400/40nm, Dichroic 430nm, Emission 444nm

ET DAPI Ex 350/50, Di 400, Em 460/50

ET-GFP Ex470/40, Di 495, Em 525/50

ET/mCH/TR  Ex560/55, Di 585, Em 630/75 

ET Cy5 Ex 620/60, Di 660, Em 700/75

More information and scheduling at www.Bookkit.org


manual 80i2047 80i upright manual fluorescent microscope

Fluorescent and Brightfield 

Objectives

1X/2X Plan UW

4x/20X Plan Fluor

10x/40X DIC

20x Plan Fluor 

Epi cubes 

UV-2E/C Excitation 360/40, Dichroic 400, Emission 460/50

HQ:F Ex480/40, Di505, Em535/50

HQ:TR Ex560/55, Di595, Em6495/75

Cy5-4040-NTE Semrock Brightline, Ex628/40, Di594 - 651 nm, 669 - 726 nm, Em692/40

More information and scheduling at www.Bookkit.org


3019 80i3019 80i upright fluorescent microscope

Brightfield, Darkfield, and Fluorescence

Objectives

Plan Apo 2X/4X/10X/20X/40X/60X

Epi cubes

UV-2E/C  Ex 360/40 Di 400 Em 460/50

HQ:F Ex480/40 Di 505 Em 535/50

HQ:TR Ex560/55 Di595 Em 645/75 

Cy5-4040 -NTE Semrock Brightline Ex628/40 Di 594 - 651 nm, 669 - 726 nm Em 692 /40

More information and scheduling at www.Bookkit.org


ix713019 Olympus IX71 inverted fluorescent microscope

Fluorescence, Brightfield, and Phase contrast

Objectives

PlanAPO 2X; UPlanFl 4X/10X; LCPlanFl 20X/40X/60X

Epi cubes

UV, Excitation 350/50nm, Dichroic 400nm, Emission 420nm Long Pass

Blue GFP II, EX 380/30, Di 420, Em 460/50

EN GFP, Ex 470/40, Di 495, Em 525/50

HQ:FITC, Ex 480/40, Di 505, Em 535/50

 HQ:R/DiI, Ex 540/25, Di 565, Em 605/55

More information and scheduling at www.Bookkit.org


Stereomicroscopes


2031 Leica MZ FLII fluorescent stereomicroscope

Room 2031 is a physiology/surgical suite.  All procedures must have an approved ACUP.  This is a shared room, so please coordinate with other occupants.

The Leica MZ FL III is a fluorescent stereomicroscope with a 12.5:1 zoom

It has a mercury lamp with the following filter choices with color camera for image capture:

V-            425/40nm ex, 475nm em
GFP2-     480/40nm ex, 510nm em
RdsR-     535/50nm ex, 610/75nm em
G-           546/10nm ex, 590nm em
TxRD-    560/40nm ex, 630/60nm em

More information and scheduling at www.Bookkit.org


SMZ 15002047 SMZ1500 fluorescent stereomicroscope

Brightfield and Fluorescence

Objectives

P-HR Plan Apo 1x/1.6X

Epi cubes

Cyan (ECFP) Ex 436/20 Di 455 Em 480/40

Endow GFP Bandpass 470/40 Di 495 Em 525/50

TRITC (DsRed) Ex 545/30 Di 570 Em 620/60

Camera

Nikon color Digital Sight DS-2Mv with a DS-U2 controller

More information and scheduling at www.Bookkit.org


High Content Microscope


2051 High Content Analysis fluorescent microscope

Nikon Eclipse TiE with HL20C plate loader

For brightfield or fluorescent high content imaging of multiple plates or slides contact Sarah Tague (stague@kumc.edu)


Offline workstations


High Content2047 High Content Analysis Computer

 Zenith Workstation - HP Z840

Processor dual Intel Xeon 3.5 GHz processors (8 cores toal)
Memory- 256GB DDR4 2400 Mhz RAM 

Video-GTX 1080 8GB GDDR5XGPU

Storage- 1TB Turbo SSD for OS, 6TB (3GBx2 Raid 0) data storage

Screen- 34" Ultra Wide Curved Display with 3440 x 1440 resolution

Software 

Nikon NIS-Elements Advanced research, with General Analysis (Automated image anlysis) and Extended Depth of Focus. Adobe suite Stereoinvestigator (MBF Bioscience) Cellprofiler Image J/ FIJI

More information and scheduling at www.Bookkit.org


2047 Common PC Workstation

Software

Adobe suite (Photoshop, Illustrator, InDesign)

Microsoft Office

ImageJ / FIJI

This workstation does not have Nikon NIS-Elements.

More information and scheduling at www.Bookkit.org