NovaSeq X Plus

The following is pricing for NovaSeq X Plus full flow cell runs or single lane flow cell runs. Partial lane multiplexed sequencing cost is based on the percent consumption of the available reads for the flow cell lane used. 

The KIDDRC Bioinformatics Core on the University of Kansas Medical Center Campus is available to provide sequence data analysis services. Please contact Sumedha Gunewardena sgunewardena@kumc.edu for service information.

1.5B Flow Cell - 10B Flow Cell Pricing

26B Flow Cell Pricing

For Full Flow Cell Run Pricing, please direct inquiries to: 

Sequence Library Preparation

Library Description

Tecan Universal Plus mRNA-seq Library w/ UDI – Employs oligo dT enrichment of messenger RNA to assess expression including alternative transcripts and gene fusions. Provides strand of origin to detect antisense transcription. Unique Dual Index (UDI) adapter compatible.

• Input range of total RNA 10ng – 1ug. Targeted input 1ug.
• Sequencing recommendation – 25M 100bp paired end reads*.

Illumina Stranded Total RNA Library – Includes Ribosomal reduction to enrich for mRNA and non-coding transcript interrogation. Provides strand of origin to detect antisense transcription. Compatible with FFPE RNA. Unique Dual Index (UDI) adapter compatible. DNase treatment of the RNA is mandatory for Stranded Total RNA library preparation.

• Input range of total RNA 100ng – 1ug. Targeted input 1ug.
• Sequencing recommendation – 40M 100bp paired end reads*.

Illumina RNA Prep + Enrichment, (L) Tagmentation Library – Couples reverse transcription of RNA to dscDNA and On-Bead Tagmentation with an enrichment panel (Illumina Exome Panel or Respiratory Virus Oligo Panel v2 w/ SARS-CoV-19) to capture coding transcriptome/RNA exome.  Compatible with low yield, low quality and FFPE total RNA. Targeted input variable based on RNA quality. Unique Dual Index (UDI) compatible.

• Targeted input of total RNA 10ng - 100ng. Input range of low-quality RNA or FFPE RNA 20ng – 100ng (%DV200 ≥ 36.5%).
• Sequencing recommendation – 25M 100bp paired end reads*.

QIAseq miRNA Library – Enables library preparation of any miRNA with a 3’ hydroxyl and a 5’ monophosphate present from Dicer/Drosha processing. Used to find novel miRNAs, characterize variation with single base resolution and analyze differential expression.

• Targeted input – 100 -150ng total RNA or 10 - 50ng miRNA from extracellular vesicles.
• Sequencing recommendation – 5M  reads*.

Illumina DNA Prep Library – Uses Bead-Link transposome for whole genome sequencing. Broad application range including multi-species genomes, Microbial genomes, amplicons, plasmids. Unique Dual Index (UDI) adapter compatible.

• Targeted input of high MW gDNA: large genomes 100 - 500ng / small genomes 10 - 500ng
• Sequencing recommendation – 30 X sequencing coverage*.

ChIP-Seq / CUT&RUN Library – Used as an epigenetic analysis tool, chromatin immunoprecipitation (ChIP) or CUT&RUN sequencing libraries leverage next-generation sequencing to determine the distribution and abundance of DNA bound protein targets of interest across the genome or the open chromatin of a tissue or cellular state. Fragmented control input chromatin and immunoprecipitated chromatin or enriched open chromatin are provided by the investigator. A gel image of the high MW chromatin and fragmented chromatin are required for project submission (fragmented size range 600bp – 100bp).

• Targeted input of fragmented chromatin: control input – 20ng, immunoprecipitated chromatin – 20ng. Targeted input of CUT&RUN chromatin - 10- 20ng.
• Sequencing recommendation:  30M – 40M reads* determined by predicted genome distribution of target protein.
• Sequencing recommendation for input chromatin – 45M reads.
• Sequencing recommendation for CUT&RUN chromatin - 5-10M reads

Illumina DNA Prep for Enrichment – Used for targeted resequencing in human applications. Indexed DNA libraries are prepared for pooled enrichment using exome capture probes targeting 45Mb of coding sequence from >98% of RefSeq, CCDS and Ensemble coding content. Open enrichment compatibility allows the use of non-Illumina capture probe sets allowing the expansion of the species applications available for targeted exome sequencing. Unique Dual Index (UDI) adapter compatible.

• Targeted input of intact, high MW gDNA - ≥100ng.
• Sequencing recommendation – 50M 100bp paired end reads* for human, 50X coverage (4Gb of data).

Ovation RRBS Methyl-seq Library w/ oxBS Module  – Reduced Representation Bisulfite Sequencing (RRBS) is used to generate single base resolution DNA methylation information across a genomic sample. By analyzing a reduced representation of the genome, the amount of sequencing required is greatly reduced relative to whole genome bisulfite sequencing (WGBS). The TrueMethyl oxBS module allows the differential interrogation of both 5-hydroxymethylcytosine (5hmC) and 5-methylcytosine (5mC) and provides a method to accurately quantify the true level of cytosine methylation through the subtractive analysis of two parallel library sequence data sets, one with oxidative conversion of 5hmC and one without. 

• Targeted input – 100ng RNase treated high MW gDNA.
• Sequencing recommendation – 55M 100bp paired end reads*.
• Determination of 5mC and 5hMC modified cytosine requires two independent library interrogations per sample.

*Read counts and X coverage can be modified to meet specific project demands.