The Genomics Core has established an inter-core cooperative with the KIDDRC Integrated Imaging Core and KIDDRC Histology Core to provide a complete spatial genomics service using the 10x Genomics Spatial Gene Expression system. The Visium HD workflow is supported by the 10x Genomics CytAssist tissue managment instrument for tissue placement on the Visium slide. The 10x Visium HD supports spatial interrogation of human and mouse tissues using the Visium Human Transcriptome or Visium Mouse Transcriptome probe sets to capture and amplify the spatial expression profiles. Visium HD is compatible with FFPE, fresh frozen or fixed frozen tissue (sequencing demand varies for the state of tissue used).

The Genomics Core, Intergrated Imaging, and Histology Cores also support the Visium HD 3' Spatial Expression system with polyA based capture and CytAssist tissue placement for species agnostic  interrogation of fresh frozen and H&E stained eukaryotic tissue at 2uM resolution. Tested species include human, mouse, rat, pig, rhesus, zebrafish, xenograft (human/mouse) and (rat/human). A full listing of tested tissues can be found HERE. 

For Visium HD and HD 3' project planning and preparation please contact:

Chris Smoyer - csmoyer@kumc.edu - for tissue prep, tissue imaging and CytAssist tissue placement on Visium HD slide.

Clark Bloomer - cbloomer@kumc.edu - for RNA isolation and %DV200 determination, spatial library preparation and sequencing.

Supporting documentation and video links for Visium HD tissue preparation and project planning.

Visium HD Whole Transcriptome (human or mouse) FFPE tissue preparation handbook

Visium HD Whole Transcriptome (human or mouse) fresh frozen tissue preparation handbook

Visium HD Whole Transcriptome (human or mouse) fixed frozen tissue preparation handbook

Visium HD 3' fresh frozen tissue preparation handbook 

Video: Visium HD 3' assay webinar

Grant application resources for Visium spatial gene expression

10x Visium Library Pricing*

The Pricing for 10x Visium HD & Visium HD 3' library preparation does not include sequencing on the NovaSeq X Plus. Sequencing cost is determned by the library type ( HD or HD 3' ), the preparation method of the tissue being processed ( FFPE, fresh frozen, fixed frozen), and the percent coverage of the tissue within each fiducial frame of the Visium HD slide. 

*The Genomics Core's cost for Visium HD & Visium HD 3' library preparation does not include the KIDDRC Integrative Imaging and Histology Core's costs for the tissue preparation, CytAssist tissue placement on Visium Slide, and first strand cDNA prepearation. Please contact Chris Smoyer, csmoyer@kumc.edu , for the costs associated with this portion of your Visium project workflow.