Protocols & Guides

FACSAria IIIu Dye Selection Guide

Choose only one dye for each PMT.
Please discuss your choices with flow core personnel as not all combinations are workable.

Excitation Laser	Detector	Bandpass Filter	Filter nm	Dye
Violet 405 nm	PMT A	740 LP	780/60	Dye for PMT A, Violet 405 nm
	PMT B	670 LP	705/70	Dye for PMT B, Violet 405 nm
	PMT C	630 LP	640/40	Dye for PMT C, Violet 405 nm
	PMT D	595 LP	605/40	Dye for PMT D, Violet 405 nm
	PMT E	505 LP	525/50	Dye for PMT E, Violet 405 nm
	PMT F		450/50	Dye for PMT F, Violet 405 nm

Blue 488 nm	FSC Diode		488/10	Forward Scatter
	PMT A	685 LP	695/40	Dye for PMT A, Blue 488 nm
	PMT B	505 LP	530/10	Dye for PMT B, Blue 488 nm
	PMT C		488/10	Side Scatter

Green 552 nm	PMT A		780/40	PE-Cy7
	PMT B	685 LP	710/50	PE-Cy5.5
	PMT C	635 LP	670/30	Dye for PMT C, Green 552 nm
	PMT D	600 LP	610/20	Dye for PMT D, Green 552 nm
	PMT E		575/26	Dye for PMT E, Green 552 nm

Red 637 nm	PMT A	750 LP	780/60	Dye for PMT A, Red 637 nm
	PMT B	685 LP	710/50	Dye for PMT B, Red 637 nm
	PMT C		660/20	Dye for PMT C, Red 637 nm