Cryo-EM Research Facility Personnel
David Ingham, Ph.D.
The Cryo-Electron Microscopy Facility at KUMC is managed by Dr. David Ingham. David completed his PhD in Molecular, Cellular, and Developmental Biology at the University of Kansas where he then went on to complete his postdoc training. During his graduate work David discovered he had a passion for transmission electron microscopy and how it can be used to study the relationship between protein structure and function in neurodegenerative disorders. After completing multiple research projects utilizing negative stain electron microscopy, he became interested in how single particle cryo-EM can be used to solve macromolecular structures. Under the mentorship of Dr. Erik Lundquist, David continued to use microscopy as a tool to study developmental neurobiology, with a primary focus on studying neuronal migration using light microscopy, but ultimately returned to his passion for electron microscopy when he joined KUMC in the Spring of 2023. David has completed a Facility Manager Internship at the Pacific Northwest Cryo-EM Center in Portland Oregon. He oversees all aspects of Cryo-EM at KUMC and can assist with training, sample preparation, screening, and data collection, as well as data analysis.
Bret Freudenthal, Ph.D.
Scientific Director of the KU Medical Center Cryo-EM Facility
Dr. Bret D. Freudenthal is an Associate Professor at the University of Kansas Medical Center. He is a member of the Department of Biochemistry and Molecular Biology and is affiliated with the Department of Cancer Biology. The overarching goal of his research is to understand the interplay between DNA damage and deleterious human health outcomes. The Freudenthal lab utilizes a reductionist approach consisting of structural, biochemical, cellular, and molecular biology assays to investigate complex biological questions. Dr. Freudenthal is an established member of the cryo-EM community and has successfully integrated cryo-EM into his research program over the past 5 years with the publication of several studies using cryo-EM to study DNA base excision repair. Dr. Freudenthal provides scientific and operational guidance to the cryo-EM facility as well as consultation for investigators looking to establish cryo-EM into their research program.