Sperm Cryopreservation

Responsibilities of the Investigator

  • Provide the facility with one or two males (2-4 months old) for each line.
  • Important: Each male must be of proven fertility prior to sperm freezing.
  • If you require that sperm from more than one male be frozen individually (rather than pooled), submit a requisition for each male as a separate line.
  • Although we request a 2-4 month old male, we can process any strain or age of mouse; however, it should be noted that age and mouse strain will affect the fertility of the recovered sperm.
  • Submit a Request Form Sperm Cryo to initiate your project.

Services Provided by the Facility

The facility is employing the technique for sperm cryopreservation published by the Jackson Laboratory (2008):

  • Sacrifice male(s), harvest epididymides and collect sperm in cryoprotectant medium.
  • Sperm will be pooled from all males submitted for a line.
  • Freeze and store 15 straws in liquid nitrogen.
  • Thaw a straw to determine the motility of frozen-thawed sperm.

Fee for Sperm Cryopreservation Service

$200.00 per line.

REQUEST FORM SPERM CRYO

Name of PI:
Name of Lab Contact:
Email:
Phone:
Account Number:
ACUP #:
Animal Room Bldg and #:
Name of Mouse Line:
Number of Males Submitting:
Age of Males:

Prior to submitting males for sperm cryopreservation, please mate each male to determine fertility.

Comments:

Last modified: Jan 11, 2012
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