ES Cell Karyotyping

Responsibilities of the Investigator

  • Provide the facility with a frozen vial of ES cells or a plate of growing cells.
  • Provide a protocol for culture of your ES cells.
  • Submit a Request Form in iLabs to initiate your project.

Services Provided by the Facility

The Mouse Embryonic Stem Cell Core of the Transgenic and Gene Targeting Facility conducts a simple chromosome counting procedure to determine the percentage of euploid cells.  No advanced karyotypic analysis is offered.

  • The facility will culture and arrest embryonic stem cells in metaphase with colcemid.
  • We will prepare and count 40 chromosome spreads following a basic Giesma staining protocol.
  • The percentage of euploid cells will be provided to the investigator.

Fee for Karyotyping Service

  • $300 for ES cell karyotyping per clone.

Last modified: Dec 15, 2016
Contact Us

Phone: 913-588-7113

Email: TGIF@kumc.edu

Submit request in iLabs

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