Skip redundant pieces
 

Genotyping

Responsibilities of the Investigator

  • Provide the tail biopsies uniquely labeled and a positive control (either genomic DNA positive for the mutation or the plasmid used to generate the transgenic).
  • Provide a proven PCR assay protocol and primers (100ul at 10mM concentration).
  • Alternatively, provide the sequence of your target gene, and we will design a PCR reaction.
  • Submit a Request Form Geno to initiate your project.

Services Provided by the Facility

  • The facility will prep genomic DNA from tail biopsies using the REDExtract-N-Amp Tissue PCR kit (Sigma, cat # XNATS).
  • The facility will perform a PCR for detecting the transgene(s) of interest. For an additional fee, a second PCR (GDPH, a housekeeping gene) can be performed to analyze the quality of genomic DNA.
  • A report is provided to the investigator with gel documentation of the PCR results.
  • If requested, the facility will select primers and optimize the PCR reaction conditions for an additional fee.

Fees for Genotyping Service

  • $5.00/biopsy for genomic DNA preparation and PCR analysis (a minimum of $20.00 per requisition).
  • $2.50/sample for any additional PCR analysis.
  • $100 set up fee to establish a suitable PCR assay if proven PCR conditions are not provided.

REQUEST FORM GENOTYPING

Name of PI:
Name of Lab Contact:
Email:
Phone:
Account Number:
ACUP #:
Animal Room Bldg and #:
Name of Gene:
Number of Tail Samples:
Background Strain of ES Cell Line:

Submit labeled tail biopsies to the -20o freezer in Hemenway G021.  Provide primers and a proven PCR protocol or the sequence of your gene of interest.  Please also provide a positive control.
Comments:

     Last modified: Jan 11, 2012