ES Cell Karyotyping

Responsibilities of the Investigator

  • Provide the facility with a frozen vial of ES cells or a plate of growing cells.
  • Provide a protocol for culture of your ES cells.
  • Submit a Request Form Karyo to initiate your project.

Services Provided by the Facility

The Embryonic Stem Cell Core of the Transgenic and Gene-Targeting Facility conducts a simple chromosome counting procedure to determine the percentage of euploid cells.  No advanced karyotypic analysis is offered.

  • The facility will culture and arrest embryonic stem cells in metaphase with colcemid.
  • We will prepare and count 40 chromosome spreads following a basic Giesma staining protocol.
  • The percentage of euploid cells will be provided to the investigator.

Fee for Karyotyping Service

  • $300 for ES cell karyotyping per clone.

REQUEST FORM KAYRO

Name of PI:
Name of Lab Contact:
Email:
Phone:
Account Number:
ACUP #:
Animal Room Bldg and #:
Name of ES Cell Line:
Source of ES Cell Line:
Background Strain of ES Cell Line:

Submit a vial of frozen cells or a plate of growing cells, along with a protocol for culture.

Comments:

Last modified: Jan 11, 2012
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