Sperm Cryopreservation

Responsibilities of the Investigator

• Provide the facility with one or two males (2-4 months old) for each line.
• Important: Each male must be of proven fertility prior to sperm freezing.
• If you require that sperm from more than one male be frozen individually (rather than pooled), submit a requisition for each male as a separate line.
• Although we request a 2-4 month old male, we can process any strain or age of mouse; however, it should be noted that age and mouse strain will affect the fertility of the recovered sperm.
• Submit a Request Form in iLabs to initiate your project.

Services Provided by the Facility

The facility is employing the technique for sperm cryopreservation published by the Jackson Laboratory (2008):

• Sacrifice male(s), harvest epididymides and collect sperm in cryoprotectant medium.
• Sperm will be pooled from all males submitted for a line.
• Freeze and store 15 straws in liquid nitrogen.
• Thaw a straw to determine the motility of frozen-thawed sperm.