Embryo Cryopreservation

Responsibilities of the Investigator

  • Have a minimum of five proven stud males available for each line.
  • If providing female mice, donors must be between three and four weeks of age, provided in groups of at least five.
  • Submit a Request Form Embryo Cryo to initiate your project.

Services provided by the facility

Embryo cryopreservation will be accomplished by mating donor female mice to stud males of the line of interest. The facility can purchase wildtype females of either outbred or inbred background, or the investigator can provide the donor females for a reduced fee. (Required if cryopreserving homozygous mice.)

  • The facility will superovulate donor female mice and mate to investigator stud males.
  • Embryos will be harvested at d 3.5.
  • The facility will freeze morula/blastocyst stage embryos in straws with a controlled rate freezer.
  • Store straws in liquid nitrogen.
  • Thaw test straw to confirm viability of embryos.
  • We guarantee a minimum of 250 embryos frozen or three freezing sessions.

Fees for Embryo Cryopreservation Service

  • $1200 if investigator provides donor females.
  • $1500 if facility purchases outbred donor females.
  • $2000 if facility purchases inbred donor females.
  • $300 per freezing session if investigator provides embryos.

REQUEST FORM EMBRYO CRYO

Name of PI:
Name of Lab Contact:
Email:
Phone:
Account Number:
ACUP #:
Animal Room Bldg and #:
Name of Mouse Line:
Source of Studs Available:
Source of Donor Females:

Strain of Donor Females:

Comments:

Last modified: Jan 11, 2012
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