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PCR Genotyping

Responsibilities of the Investigator

  • Provide the tail biopsies uniquely labeled and a positive control (either genomic DNA positive for the mutation or the plasmid used to generate the transgenic).
  • Provide a proven PCR assay protocol and primers (100ul at 10mM concentration).
  • Alternatively, provide the sequence of your target gene, and we will design a PCR reaction.
  • Submit a Request Form in iLabs to initiate your project.

Services Provided by the Facility

  • The facility will prep genomic DNA from tail biopsies using Qiagen's DNeasy Blood & Tissue Kit. 
  • The facility will select primers and optimize the PCR reaction conditions.
  • The facility will perform a PCR for detecting the transgene(s) of interest. 
  • A report is provided to the investigator with gel documentation of the PCR results.

Last modified: Nov 05, 2019
Contact Us

Phone: 913-588-7113

Email: TGIF@kumc.edu

Submit request in iLabs

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