Chimeric Mouse Production

Responsibilities of the Investigator

  • Obtain IACUC approval for your project before requesting service.
  • Discuss cell source with facility directors before beginning project.
  • Request karyotyping of your cells prior to injection:  Request Form Karyo
  • Prepare cells the morning of injection and submit a single cell suspension on ice, or have the facility culture cells for you.
  • Develop a reliable genotyping assay (PCR, Southern, or both) for identifying positive mice from tail clippings or ear punches.
  • Testing for germline transmission is the responsibility of the investigator.
  • Submit a Request Form Blast to initiate your project.

Services Provided by the Facility

  • When requested, the facility will expand (Request Form Exp) and prepare cells for injection.
  • Previously selected targeted ES clones will be microinjected into C57Bl/6 blastocysts, after which they will be implanted into pseudopregnant females.
  • We guarantee to inject and implant at least 60 blastocysts.
  • We cannot guarantee the production of chimeric mice, nor their germline potential.

Fee for Chimeric Mouse Production Service

  • $2500.00 will be charged after at least 60 blastocysts are injected and implanted.

REQUEST FORM BLAST

Name of PI:
Name of Lab Contact:
Email:
Phone:
Account Number:
ACUP #:
Animal Room Bldg and #:
Name of ES Cells:
Source of ES Cells:
Background Strain of ES Cells

Submit passaged ES cells in single cell suspension from 6-well plate in 3ml medium on ice the morning of scheduled injection.

Comments:

Last modified: Feb 10, 2012
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