Transgenic and Gene Targeting Institutional Facility

Welcome to the Transgenic and Gene Targeting Institutional Facility at the University of Kansas Medical Center! We are an institutional research support facility providing a centralized service for the production of novel transgenic and gene targeted rodents and pluripotent stem cells for the investigators of KUMC and the surrounding Kansas City research community. Our core uses cutting edge technologies, including CRISPR/Cas9 genome editing, for the generation of these models. Please click on the links to the left for specific descriptions of these services.
 

Transgenic Mouse: Methods and Protocols

Edited by Melissa A. Larson, Ph.D., Technical Director of the Transgenic and Gene Targeting Institutional Facility at the University of Kansas Medical Center.

This new book, part of the Springer Methods in Molecular Biology Series, contains comprehensive information about a variety of topics related to transgenic mouse production. Listed below are several chapters authored by KUMC faculty and staff.

Pronuclear Microinjection of One-Cell Embryos
Melissa A. Larson

In Vivo Validation of CRISPR Reagents in Preimplantation Mouse
Melissa A. Larson, Katelin A. Gibson, and Jay L. Vivian

Blastocyst Microinjection with Embryonic Stem Cells
Melissa A. Larson

Embryo Transfer Surgery
Melissa A. Larson

Strategies for Behaviorally Phenotyping the Transgenic Mouse
Kenneth E. McCarson

Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells Using Sendai Virus
Julia M. Draper and Jay L. Vivian
 

Our Support

The Transgenic and Gene Targeting Institutional Facility receives support from a variety of institutional and NIH funded programs.  Users of the Facility should acknowledge the relevant support in all publications using TGIF services.

• University of Kansas Medical Center (Peter Smith PhD, Senior Associate Dean for Research)  
• Center of Biomedical Research Excellence (COBRE) Program Project in Molecular Regulation of Cell Development and Differentiation (NIH P30 GM122731, Dale Abrahamson PhD, PI)  
• University of Kansas Cancer Center (NIH P30 CA168524, Roy Jensen MD, PI)  
• Kansas Intellectual and Developmental Disabilities Research Center (KIDDRC; NIH U54 HD090216; John Colombo PhD, Director, Peter Smith PhD, Co-Director)    
• Kansas IDeA Network of Biomedical Research Excellence (K-INBRE; P20GM103418, Doug Wright PhD, PI)

CONTACT US: Phone 913-588-7113; TGIF@kumc.edu