Ke (Michael) Liu, PhD

Postdoctoral Fellow
PhD in Bioanalysis and Drug Metabolism, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China, 2009

I recieved my Ph.D. degree in bioanalysis and drug metabolism from Shanghai Institute of Materia Medica, Shanghai, China, 2009. My Ph.D. dissertation was focused on enantioselective LC-MS/MS assays of chiral drugs (ondansetron, doxazosin and leucovorin), enantioselective drug metabolism and pharmacokinetics. After graduation, I began working at the Center for Drug Metabolism Research, Shanghai Institute of Materia Medica as a research assistant professor from 2009 to 2011. My research was focused on the bioanalysis using LC-MS/MS, pharmacokinetics and metabolism of drugs or drug candidates in pre-clinical and clinical trials. From 2011 to 2012, I worked for Dr.Robert Hanzlik as a postdoctoral fellow in reactive metabolite toxicity field in Department of Medicinal Chemistry, University of Kansas. At the end of 2012, I joined the laboratory of Dr.Ma as a postdoctoral fellow.

Research Interests
Drug metabolism, Drug induced liver injury, Metabolomics, Bioanalysis

My research focuses on mechanisms of hepatotoxicity caused anti-tuberculosis (TB) drugs. The role of CYP3A and PXR in anti-TB drug-induced liver injury is investigated in genetically engineered PXR and CYP3A mouse models. An LC-MS-based metabolomic approach is used to profile: (1) metabolism and bioactivation of anti-TB drugs and (2) biomarkers of anti-TB drug-induced liver injury.

Selected Publications

Yakov M. Koen, Heather Hajovsky, Ke Liu, Todd D. Williams, Nadezhda A. Galeva, Jeffrey L. Staudinger and Robert P. Hanzlik. Liver protein targets of hepatotoxic 4-bromophenol metabolites. Chem. Res. Toxicol., 2012, 25(8):1777-1786.

Yong Yang, Ke Liu, Dafang Zhong, Xiaoyan Chen. Simultaneous determination of flumatinib and its two major metabolites in plasma of chronic myelogenous leukemia patients by liquid chromatography-tandem mass spectrometry. Journal of Chromatography B, 2012, 895-896, 25-30

MeiXia Chen, Ke Liu, Dafang Zhong, Xiaoyan Chen. Trimethylsilyldiazomethane derivatization coupled with solid-phase extraction for the determination of alendronate in human plasma by LC-MS/MS. Analytical and Bioanalytical Chemistry. 2012 402(2):791-798.

Ke Liu, Dafang Zhong, Xiaoyan Chen. Enantioselective determination of doxazosin in human plasma by liquid chromatography-tandem mass spectrometry using ovomucoid chiral stationary phase. Journal of Chromatography B. 2010, 878, 2415-2420

Ke Liu, Dafang Zhong, Huiyu Zou, Xiaoyan Chen. Determination of tegafur, 5-fluorouracil, gimeracil and oxonic acid in human plasma using liquid chromatography-tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis. 2010, 52, 550-556.

Ke  Liu,  Xiaojian  Dai,  Dafang  Zhong,  Pan  Deng,  Jinfei  Ma,  Xiaoyan  Chen.  Simultaneous  determination  of6R-leucovorin, 6S-leucovorin and 5-methyltetrahydrofolate in human plasma using solid phase extraction and chiral liquid chromatography-tandem mass spectrometry. Journal of Chromatography B. 2009, 877, 902-910.

Ke Liu, Dafang Zhong,  Xiaoyan Chen. Enantioselective quantification of chiral drugs in human plasma with LC-MS/MS. Bioanalysis (review). 2009, 1, 561-576.

Ke Liu, Xiaojian Dai, Dafang Zhong, Xiaoyan Chen. Quantitative determination of ondansetron in human plasma by enantioselective  liquid  chromatography-tandem  mass  spectrometry. Journal  of  Chromatography  B.  2008, 864,129-136.

Ke Liu, Linqi Yan, Guocan Yao, Xingjie Guo. Estimation of p-coumaric acid as metabolite of E-6-O-p-coumaroyl scandoside  methyl  ester  in  rat  plasma  by  HPLC  and  its  application  to  a  pharmacokinetic  study.  Journal  of Chromatography B. 2006, 831, 303-306.