Graduate Student - Lampe Lab
B.A.. in Biology, University of Kansas
M.A. in Biochemistry and Biophysics, University of Kansas
My research in the Lampe lab has two primary foci: 1) developing novel click chemistry-based approaches for investigating protein-protein interactions and 2) investigating the survivin protein structure and function to facilitate rational design of small molecule inhibitors.
Click chemistry, initially described in 2001 by Nobel laureate K. Barry Sharpless, provides a facile approach to performing specific synthetic chemical reactions under physiologically-relevant (aqueous) conditions. These synthetic chemical reactions, along with site-directed mutagenesis approaches, can facilitate the site-specific incorporation of a lanthanide binding tag into a protein of interest. Lanthanides are a class of metals that, because of their unique f-orbitals, have strong luminescent properties. Capitalizing on endogenous and introduced fluorescent properties has been a popular approach for studying protein-protein interactions, but, because of the inability to direct the placement of the fluorophore and the infinitesimal duration of the fluorescent lifetime, their utility can be limited. We therefore plan to capitalize on the lanthanides' luminescent properties to study protein-protein interactions on a longer timescale than those facilitated with fluorescent approaches. Using click chemistry and site-directed mutagenesis approaches, we can improve subsequent molecular dynamics investigations.
Despite recent, unparalleled advances in anti-cancer therapeutics, cancer remains a devastating illness for millions of people, worldwide. Survivin is a small protein that has been found to be upregulated in multiple cancers and is therefore a promising therapeutic target. As its name implies, survivin inhibits apoptosis, however, the mechanism(s) of this inhibition remains unknown. Using the aforementioned click chemistry approach, coupled with NMR, we aim to utilize structure activity relationship studies to facilitate the development of small molecule survivin inhibitors. We anticipate that this approach will facilitate rational design of small molecule inhibitors to therapeutic targets within and outside of the anti-cancer sphere.
Previously, the Madison and Lila Self Graduate Program provided four years of academic support as well as a stipend and leadership development program. Administered through the KU Lawrence campus, but open to students at both campuses, interested parties may learn more about this opportunity at the following website: (http://www2.ku.edu/~selfpro/). Graduates of the development program continue to benefit from involvement as members of The Society of Self Fellows (http://www2.ku.edu/~selfpro/socyear.shtml), which provides continued networking opportunities.Please contact me if you have questions about my research! I look forward to hearing from you!
Sullivan BP, Kopec AK, Joshi N, Cline H, Brown JA, Bishop SC, Kassel KM, Rockwell C, Mackman N, Luyendyk JP, Hepatocyte tissue factor activates the coagulation cascade in mice, Blood, 2013 Mar 7;121(10):1868-74. doi: 10.1182/blood-2012-09-455436. Epub 2013 Jan 10.Ki Sung Kang, Yujing Wen, Noriko Yamabe, Masayuki Fukui, Stephanie C. Bishop, and Bao Ting Zhu, Dual Beneficial Effects of (-)-Epigallocatechin-3-Gallate on Levodopa Methylation and Hippocampal Neurodegeneration: In Vitroand In Vivo Studies, PLoS One, Vol. 5, Issue 8, 2010, 1 - 14.
Stephanie C. Bishop, Long primer extension by a novel inverse PCR method, Master's Thesis, 2009.
Stephanie C. Bishop, Joseph A. Burlison, and Brian S. J. Blagg, Hsp90: a Novel Target for the Disruption of Multiple Signaling Cascades, Current Cancer Drug Targets, Vol. 7, No. 4, 2007, 369 - 388.
Ellen E. Quillen, Gale C. Haslam, Hardeep S. Samra, Darius Amani-Taleshi, Jeffrey A. Knight, Diane E. Wyatt, Stephanie C. Bishop, Kim K. Colvert, Mark L. Richter, and Paul A. Kitos, Ecto-adenylate kinase and plasma membrane ATP synthase activities of human vascular endothelial cells, Journal of Biological Chemistry, Vol. 281, No. 30, 2006, 20728 - 20737.
Graduate Student - Lampe Lab
4023 HLSIC, MS 1018
3901 Rainbow Blvd.
Kansas City, Kansas 66160
F: (913) 588-7501