Quantitative PCR (qPCR or Real-Time PCR)

INSTRUMENT NAME

FEATURE

PHOTO

BioRad CFX384 qPCR System
(HLSIC: 4017)

Multiplex qPCR system featuring

  • 384-well plate format qPCR

  • 5-20 µL sample size

  • Excitation/emission range: 450-690 nm

  • CFX Manager software

BioRad cfx384

BioRad CFX384 qPCR System

 Thermo Scientific (Applied Biosystems)
  StepOnePlus qPCR System
(HLSIC: 4005: Jointly administered by the ACL and Dr. Michele Pritchard)
  • 96-well plate format qPCR
  • 10-40 µL sample size
  • Dye compatibility: FAM/SYBR, VIC/JOE, ROX, NED/TAMRA
  • StepOnePlus Software v2.3
Thermo Scientific (Applied Biosystems) StepOnePlus qPCR System
 Agilent 2100 Bioanalyzer System
(HLSIC: 4017)
  • Easy to use - load the chip, press "start"
  • Ready-to-use chip based capillary electrophoresis assays and pre-packaged reagent kits
  • Minimal sample consumption (1-4 µL)
  • 25-45 minute analysis time
  • 11-12 Samples per chip*

Compatible Kits Available from Agilent for DNA/RNA Quantitation*

  • DNA 1000 Kit (Cat# 5067-1504; )
    Sizing Range: 100-1000 bp. Sensitivity: 0.5-50 ng/µL. Kit: 25 Chips, 12 Smpl/chip
  • DNA 7500 Kit (Cat# 5067-1506)
    Sizing Range: 100-7500 bp. Sensitivity: 0.5-50 ng/µL. Kit: 25 Chips, 12 Smpl/chip
  • DNA 12000 Kit (Cat# 5067-1508)
    Sizing Range: 100-12000 bp. Sensitivity: 0.5-50 ng/µL. Kit: 25 Chips, 12 Smpl/chip
  • High Sensitivity DNA Kit (Cat# 5067-4626)
    Sizing Range: 50-7000 bp. Sensitivity: 5-500 pg/µL. Kit: 10 Chips, 11 Smpl/chip
  • RNA 6000 Nano Kit (Cat# 5067-1511)
    Sizing Range: mRNA/total RNA. Sensitivity: 25-500 ng/µL. Kit: 25 Chips, 12 Smpl/chip
  • RNA 6000 Pico Kit (Cat# 5067-1513)
    Sizing Range: mRNA/total RNA. Sensitivity: 50-5000 pg/µL. Kit: 25 Chips, 11 Smpl/chip
  • Small RNA Kit (Cat# 5067-1548)
    Sizing Range: 6-150 nt. Sensitivity: 50-2000 pg/µL. Kit: 25 Chips, 10 Smpl/chip

Compatible Kits Available from Agilent for Peptide & Protein Quantitation*

  • Protein 80 Kit (Cat# 5067-1515)
    Sizing Range: 5-80 kDa. Sensitivity: 60-2000 ng/µL. Kit: 25 Chips, 10 Smpl/chip
  • Protein 230 Kit (Cat# 5067-1515)
    Sizing Range: 14-230 kDa. Sensitivity: 15-2000 ng/µL. Kit: 25 Chips, 10 Smpl/chip

*Contact ACL Director. Each kit has a 4 month half-life and consequently they are purchased according to demand. ACL will purchase the kit as required and invoice the client at $5.00 per sample ($6.00 for high sensitivity DNA assays).

Agilent 2100 Bioanalyzer System
Last modified: Sep 06, 2017
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