This is a basic service designed to allow experienced and trained clients access to the instruments.
The following rules are in place to protect the instruments from preventable instruments.
ACL staff must approve the assay procedure that the client intends to run.
The ACL will provide the UPLC solvents (see below) and glassware associated with blending the mobile phase.
All blended mobile phases must be pre-filtered using either GHP or Nylon filters (provided by the ACL).
Only auto-sampler plates and vials approved by ACL staff will be permitted. The ACL is mainly concerned with about ensuring the proper clearance for the autosampler robot.
Only UPLC columns approved by ACL staff will be permitted. The ACL is mainly concerned with ensuring the column is compatible with the UPLC pumps don't damage
Mobile Phase Components that the ACL will provide as part of this service.
The following are what the client is expected to provide -- these rules keep instrument access costs to a minimum.
The client will furnish their own consumables including:
Authentic standards for calibration.
Purified compounds for use as internal standards (i.e. isotope labeled versions of the analyte).
Auto-sampler vials (subject to ACL approval)
UPLC column (subject to ACL approval).
Specialized solvents and reagents (subject to ACL approval) examples include:
Reducing agents (e.g. DTT).
Oxidizing agents (e.g. sodium hypochlorite)
Perishable acids or bases (e.g. perchloric acid)
Labeling and derivatizing agents (e.g. dansyl chloride).
Adducting agents (lithium chloride)
Ion-pairing compounds (e.g. triethylamine, triethylammonium acetate)
Mobile phase modifiers, particularly those non-volatile compounds used to modulate pH or ionic strength (e.g. hexafluoroisopropanol, TRIS).
APPI dopants (e.g. acetone, toluene)
Atypical mobile phase components (e.g. ethanol)
Atypical sample diluents (e.g. DMF, DMSO)
Mobile phase components that are corrosive to the instruments in their typical configuration (e.g. nitric acid, citric acid, THF, sodium salts of phosphate).
Matrix compounds (i.e. pooled blank plasma, liver tissue, brain tissue)
Previously the ACL provided its clients a column from the "communal column library." As of July 2017 the ACL will discontinue this policy. This new policy will simplifying the task of troubleshooting cross-contamination or fouling or physical damage meted out by previous users. It also allows the ACL to keep the price of its "instrument only access" service low.
But more importantly, having the client purchase and maintain their own columns -- reserving them for use with a particular assay or even related assays in a particular lab -- improve the batch-to-batch repeatability of the assay and the half-life of these $900 items.