This is a standardized comprehensive LC-MS assay designed to comprehenively quantify Bile acids in biological tissues or fluids.
The key features of the service offered are:
The ACL typically returns your results to you in under three weeks.
Three deuterated standards: CA, UDCA, and LCA.
8-point external calibration curve.
The ACL runs quality control samples to monitor batch-to-batch reproducibility.
The ACL can prepare your samples from tissue if required.
Solid-phase extraction used to extract BA from tissue samples -- minimizes the effect of different biological matrices.
Methods for BA analysis in human, rat and mouse. (plasma, urine, bile, intestine, liver, gall-bladder and feces.)
Bile acids currently quantified by the assay: Note ω-MCA and T-ω-MCA will be added soon. Additional BAs can be added at clients request.
CA, CDCA, DCA, LCA, UDCA, HCA, α-MCA, β-MCA,
G-CA, G-CDCA, G-DCA, G-LCA, G-UDCA,
T-CA, T-CDCA, T-DCA, T-LCA, T-UDCA. T-HCA, T-α-MCA, T-β-MCA.
Limit of quantitation: 0.5 pmol/µL
Total ion current chromatograph of a ~2 ng/µL bile acid standard.