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Amino-Acid Assay

This service is based upon the AccQ.Tag Amino Acid assay offered by Waters Corporation. a standardized comprehensive LC-assay (detection by ultra-violet spectorphotometric or fluorescence detectors) designed to comprehenively quantify proteinogenic amino acids in biological fluids, cultured cell media and food samples.

The key features of the service offered are:

  • All consumables included in the price.

  • One standard: (norvaline).

  • 8-point external calibration curve.

  • Derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) increases sensitivity and selectivity.

  • The ACL runs quality control samples to monitor batch-to-batch reproducibility.

  • The ACL can prepare your samples from tissue if required.

  • Methods for amino acid analysis in serum, urine, cultured and cell media. Techniques for tissue analysis coming soon.

  • Validated methods to extend the assay to a wider array of biogenic amines using MS, coming soon.

  • Analytes quantified by the assay:Limit of quantitation: 50 fmol/µL

    • Ala, Gly, Ile, Leu, Pro, Val, Phe

    • Trp, Tyr, Asp, Glu, Arg, His, Lys

    • Ser, Thr, Cys, Met, Asn, Gln. 

 

Example FLR Chromatogram (Waters Amino Acid Hydrolysate Standard without Norvaline)

AAA Assay

 

Last modified: Aug 13, 2018
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