This service is based upon the AccQ.Tag Amino Acid assay offered by Waters Corporation. a standardized comprehensive LC-assay (detection by ultra-violet spectorphotometric or fluorescence detectors) designed to comprehenively quantify proteinogenic amino acids in biological fluids, cultured cell media and food samples.
The key features of the service offered are:
One standard: (norvaline).
8-point external calibration curve.
Derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) increases sensitivity and selectivity.
The ACL runs quality control samples to monitor batch-to-batch reproducibility.
The ACL can prepare your samples from tissue if required.
Methods for amino acid analysis in serum, urine, cultured and cell media. Techniques for tissue analysis coming soon.
Validated methods to extend the assay to a wider array of biogenic amines using MS, coming soon.
Analytes quantified by the assay:Limit of quantitation: 50 fmol/µL
Ala, Gly, Ile, Leu, Pro, Val, Phe
Trp, Tyr, Asp, Glu, Arg, His, Lys
Ser, Thr, Cys, Met, Asn, Gln.
Example FLR Chromatogram (Waters Amino Acid Hydrolysate Standard without Norvaline)