This is a standardized comprehensive LC-MS assay designed to comprehenively quantify acetaminophen (APAP) and the products of APAP phase II metabolism in biological tissues or fluids.
The key features of the service offered are:
The ACL typically returns your results to you in under three weeks.
Two deuterated standards: (APAP and APAP-sulfate).
8-point external calibration curve.
The ACL runs quality control samples to monitor batch-to-batch reproducibility.
The ACL can prepare your samples from tissue if required.
Methods for APAP/APAP-metabolite analysis in human, rat and mouse serum. Techniques for tissue analysis coming soon.
Analytes quantified by the assay:Limit of quantitation: 0.25 pmol/µLImproved version of the assay developed for Xie et al. (2015). Run-time per sample reduced from 40 minutes to 5 minutes. This eliminates problems with analyte instability.
acetaminophen (APAP), acetaminophen N-acetylcysteine (APAP-NAC), acetaminophen glutathione (APAP-GSH)
acetaminophen sulfate (APAP-Sulf), acetaminophen glucuronide (APAP-Gluc), 3-cysteinylacetaminophen (APAP-Cys)
Total ion current chromatograph of a ~0.2 ng/µL APAP assay standard.