Equipment & Cores
Cell Isolation Core
The Cell Isolation Core is a COBRE funded lab that provides isolated hepatocytes from human liver, technical support and experimental design for research investigators at KUMC and other KU campuses for a minimal cost. Hepatocytes can be used for a variety of studies that include drug metabolism, toxicology, enzyme activity assays and treatment of metabolic disease by cell transplantation. The lab is located in room 4052 of the state-of-the-art Hemenway Life Sciences Innovation Center. The core is equipped with all of the necessary equipment and supplies for the isolation and culture of hepatocytes. Some of the equipment includes: 2 NuAire biological safety cabinets (model NU-425-400), an Eppendorf refrigerated centrifuge (model 5810R), an Eppendorf refrigerated microfuge (model 5424R), a Cole-Parmer Masterflex L/S peristaltic pump (model 7523-90), a NuAire water jacketed CO2 incubator (model NU-8700) and a VanGuard inverted microscope with camera (model 1493Ini). The Cell Isolation Core also provides snap frozen hepatocytes and liver tissue as well as RNA, DNA and protein fractions. With the cooperation of the KU Liver Center and KU Hospital, the Cell Isolation Core can provide research investigators with a link to clinical investigators that will allow for greater understanding of liver disease and how to treat it.
The Histopathology Core supports the COBRE projects by facilitating the rapid phenotype analysis of transgenic or null mice. For example, in determining gene expression pathways that are regulated in the liver of OB/SHP null mice as compared to control OB/OB and SHP null mice under different conditions. The functions of the core include determining gross histological characteristics of tissue, measuring and localizing protein and gene expression in liver or other tissues by immunocytochemistry or in situ hybridization techniques, perform serum chemistry analysis to follow liver injury and repair during studies of fibrogenesis or fatty liver, and quantitating different cell types isolated from the liver or other sources by flow cytometry.
The Analytical Core supports the investigators of the COBRE projects by providing instrumentation and expertise in its application to research problems. Specifically, the Analytical Core provides and promote HPLC, LC-MS/MS, GC, and Multi-mode spectrophotometry as analytical tools available to our investigators. This Core provides COBRE investigators with modern instrumental approaches for the isolation, identification, and quantification of compounds of relevance to their studies of liver function in health and disease. It validates instrument performance as a component of an overall quality assurance program for this core facility. This Core assists and trains COBRE investigators in appropriate sampling and sample preparation to take advantage of these instrumental approaches. Finally, the Analytical Core develops working relationships with other core facilities on this campus and on other campuses in the state to further develop the capabilities and the efficiency of all cores.