Jay L Vivian, PhD

Research Associate Professor, Division of Cancer & Developmental Biology, Department of Pathology & Laboratory Medicine
Ph.D., University of Texas-Houston, 1999
Postdoctoral, University of North Carolina at Chapel Hill, 2000-2004


Office: 3063 Hemenway 

Phone:  (913) 588-0341 

Fax: (913) 588-7180

Email: jvivian@kumc.edu

 

Laboratory: 3051 Hemenway Building

Phone:  (913) 588-0458


Academic Positions:

Research Associate Professor
Department of Pathology and Laboratory Medicine

Scientific Director, Transgenic and Gene Targeting Institutional Facility

University of Kansas Medical Center


KUMC Center/Institute Affiliations:

Member, Institute for Reproductive Health and Regenerative Medicine

Member, Center for Epigenetics and Stem Cell Biology


Research Interests:

My research uses embryonic and induced pluripotent stem cells as genetic and developmental models to study signaling during embryonic development and the regulation of pluripotency.  My group is interested in understanding the signaling pathways and genetic hierarchies that regulate gene expression and stem cell self-renewal in embryonic stem cells.  A major project seeks to understand the function of TGF-beta-related signaling pathways in stem cell self-renewal.  These efforts have uncovered important and previously uncharacterized roles for the Nodal and BMP signaling pathways in the dynamic heterogeneity that exists in pluripotent cells.  A major effort in our lab focuses on understanding heterogeneity in undifferentiated cells: how heterogeneity arises and how it may be regulated.  This basic research question has a major impact on understanding the complex phenotype of pluripotency and stem cell self-renewal.

 

A major focus of my group also involves the use of pluripotent stem cells as models of human disease.  Our work aims to develop cellular therapies for spinal cord injury using human induced pluripotent stem (iPS) cells differentiated to specific neural lineages.  This work makes use of novel engineering strategies for introducing reporters into human iPS cells to monitor directed neural differentiation, and for use in high-throughput screens.

 

My group makes substantial use of mouse embryonic stem cells for genetic engineering to generate transgenic and mutant mice, for the development of animal models of congenital diseases mapped by human genetics studies. I have a sustained interest in developing novel strategies to generate mutant and transgenic animals to support these studies. These efforts leverage our knowledge of both stem cell and developmental biology.  


Selected recent publications:

Burgess-Galvin, K.E., Travis, E.D., Pierson, K.E., and Vivian, J.L. (2013).   TGF-beta-related signaling in embryonic stem cell maintenance: self-renewal as a dynamic and regulated equilibrium.  Stem Cells 31(1):48-58.

 

Home, P, Biswarup Saha, B., Ray, S., Debasree Dutta, D., Gunewardena, S., Yoo, B., Vivian, J.L., Larson, M., Petroff, M. , Gallagher, P.G., Schulz, V., White, K.L., Thaddeus G. Golos, T.G. , Behr, B., Paul, S. (2012).  Altered Subcellular Localization of Transcription Factor TEAD4 Regulates First Mammalian Cell Lineage Commitment. PNAS 109(19):7362-7.

 

Burgess-Galvin, K.E. and Vivian, J.L. (2011).  Transforming Growth Factor-beta superfamily in mouse embryonic stem cell self-renewal. Vitamins and Hormones 87: Stem Cell Regulators. 87:341-65. PMID: 22127250.

 

Copeland JN, Feng Y, Neradugomma NK, Fields PE, Vivian JL. Notch signaling regulates remodeling and vessel diameter in the extraembryonic yolk sac. BMC Dev  Biol. 2011 Feb 25;11:12.

 

Feng Y, Yang Y, Ortega MM, Copeland JN, Zhang M, Jacob JB, Fields TA, Vivian JL, Fields PE. Early mammalian erythropoiesis requires the Dot1L methyltransferase. Blood. 2010 Nov 25;116(22):4483-91.

 

Galvin KE, Travis ED, Yee D, Magnuson T, Vivian JL. Nodal signaling regulates the bone morphogenic protein pluripotency pathway in mouse embryonic stem cells.  J Biol Chem. 2010 Jun 25;285(26):19747-56.

 

Dutta D, Ray S, Vivian JL, Paul S. Activation of the VEGFR1 chromatin domain:  an angiogenic signal-ETS1/HIF-2alpha regulatory axis. J Biol Chem. 2008 Sep 12;283(37):25404-13.

 

Kirn-Safran CB, Oristian DS, Focht RJ, Parker SG, Vivian JL, Carson DD. Global growth deficiencies in mice lacking the ribosomal protein HIP/RPL29. Dev Dyn. 2007 Feb;236(2):447-60.

 

Vivian JL, Chen Y, Magnuson, T. Gene-based screens of chemically mutagenized mouse embryonic stem cells.  In: Handbook of Stem Cells. Lanza RP, et al (Eds), Academic Press, 2004. 

 

Chen Y, Vivian JL, Magnuson T. Gene-based chemical mutagenesis in mouse embryonic stem cells. Methods Enzymol. 2003;365:406-15.

 

Vivian JL, Chen Y, Yee D, Schneider E, Magnuson T. An allelic series of mutations in Smad2 and Smad4 identified in a genotype-based screen of N-ethyl-N-nitrosourea-mutagenized mouse embryonic stem cells. Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15542-7.

Last modified: Sep 23, 2013

Contact

Jay L Vivian, PhD
Research Associate Professor, Division of Cancer & Developmental Biology, Department of Pathology & Laboratory Medicine

Pathology & Laboratory Medicine
3901 Rainbow Boulevard
Kansas City, KS 66160

P: (913) 588-0341
F: (913) 588-0385
jvivian@kumc.edu

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