Harvard University, 1975
Ovarian function, gamete biology and early mammalian development as related to contraceptive development, infertility treatments and regenerative medicine.
Our laboratory employs genetic, molecular and imaging strategies to study basic aspects of the process of reproduction that bear on human disease and its clinical management by stem cell therapy. The overall emphasis is on Women's Health in relation to causes of human infertility, ovarian cancer, and the deployment of Assisted Reproductive Technologies (ARTS) for improving egg and embryo quality in human and animal models. Three project areas are actively under study:
1.Ovarian Physiology-the basis of signaling between the somatic and germ cell compartments of the mammalian ovary is studied using mouse knockout models of TGF-beta and gap junctional communication as related to the regulation of oogenesis and folliculogenesis.
2. Oocyte Meiotic Cell Cycle Regulation-how modifications in checkpoint control of somatic cells are adapted to ensure chromosome balance during meiosis in oocytes is being approached by live cell imaging techniques that permit simultaneous visualization of microtubule and chromosome dynamics during cell cycle progression. This problem bears directly on the effects of maternal aging and environmental exposure on reproductive fitness in aging women and is especially pertinent to the cause of Trisomy 21 or Downs Syndrome.
3. Embryonic Origins of Stem Cells- the low efficiency and poor development manifest after in vitro embryo production in most mammals is thought to be the result of both maternal environment and culture conditions that influence generation of placental and embryonic progenitors. Live imaging, genetic marking, and gene expression studies are aimed at understanding these methodological deficiencies to improve the quality of embryos from which stem cells can be isolated. Cell polarity and cell cycle regulation, in addition to chromatin remodeling, are being focused on as likely targets for disruption in the normal cascade of developmental events that compromise embryogenesis and the ability of embryonic stem cells to retain self renewal and differentiative capacities.
(Publications selected from a total of 150 peer-reviewed publications)
Oogenesis (2013) Editors:Cotichhio, G., Albertini, D.F., and DeSantis, L. Springer. ... show all 3 hide ISBN: 978-0-85729-825-6 (Print) 978-0-85729-826-3 (Online); DOI 10.1007/978-0-85729-826-3
Chapters and Original Papers:
Yesilaltay A, G. Dokshina, D. Bussoc, L. Wanga, D. Galianid, T. Chavarriae, E. Vasilef,
L.Quilaqueoc, J. Orellanag, D.Walzera, R. Shalgi, N. Dekel, D.F.Albertini,A.Rigotti, D.C.Page, and M. Krieger (2014). Excess cholesterol induces mouse egg activation and may cause female infertility. Proc. Nat. Acad. Sci. (USA) www.pnas.org/cgi/doi/10.1073/pnas.1418954111
Sanfins, A., Plancha, C.E., Albertini, D.F. (2014). Pre-implantation developmental potential from in vivo and in vitro matured mouse oocytes: a cytoskeletal perspective on oocyte quality. J. Assist. Reprod. Genet. DOI 10.1007/s10815-014-0363-4.
Albertini, D.F. The Mammalian Oocyte. In, Knobil and Neill's Physiology of Reproduction, Editors-in-Chief Tony M. Plant and Anthony J. Zeleznik, 4th edition doi.org/10.1016/B978-0-12-397175-3.00002-8
Franciosi, F., Coticchio, G., Lodde, V., Tessaro, I., Modina, SC., Fadini, R., Dal Canto, B., Renzini, MM., Albertini, DF., Luciano, AM. (2014). Natriuretic Peptide Precursor C Delays Meiotic Resumption and Sustains Gap Junction-Mediated Communication in Bovine Cumulus-Enclosed Oocytes. Biol. Reprod. 91:1-9. DOI 10.1095/biolreprod.114.118869
Coticchio, G, Gugliemo, M.C., Albertini, D.F., Dal Canto, M., Mignini Renzini, M. De Ponti, E. Fadini, R. (2014). Contributions of the actin cytoskeleton to the emergence of polarity during maturation in human oocytes. Molec. Human Reprod. 20:200-207. (10.1093/molehr/gat085)
Anderson, R.A., McLaughlin, M., Wallace, W.H.B., Albertini, D.F., Telfer, E.E. (2014). The immature human ovary shows loss of abnormal follicles and increasing follicle developmental competence through childhood and adolescence. Human Reprod. 29: 97-106. (10.1093/humrep/det388)
Dieci, C, Lodde, V., Franciosi, F., Albertini, D.F., Lazzari, G., Galli, C., and Luciano, A.M. (2013).The effect of cilostamide on gap junction communication dynamics,chromatin remodeling, and competence acquisition in pig oocytes following parthenogenetic activation and nuclear transfer. Biol. Reprod. 89:68-75. ( DOI:10.1095/biolreprod.113.110577)
Coticchio, G. Gugliemo, M.C., Dal Canto, M., Fadini, R., Renzini, M., DePonti, E., Brambillasca, F., and Albertini, D.F. (2013). Mechanistic foundations of the metaphase II spindle of human oocytes matured in vivo and in vitro. Human Reprod. 28:3271-3282. (10.1093/humrep/det381)
Fulka, Jr., J., Langerova, A., Loi, P., Ptak, G., Albertini, D.F., and Fulka, H. (2013) The ups and downs of somatic cell nucleus transfer (SCNT) in humans. J. Assist. Reprod. Genetics 30:1055-8. ( DOI 10.1007/s10815-013-0053-7)
Lamar, C., Taymans, S., Rebar, R., LaBarbera, A., Albertini, D.F., and Gracia, C. (2013). Ovarian Reserve: Regulation and Implications for Women's Health. Proceedings of the 2012 NICHD-ASRM Conference. 2013. J. Assist. Reprod. Genetics 30: 285-292.
Li, R and Albertini, DF ( 2013) The road to maturation: somatic cell interaction and self-organization of the mammalian oocyte. Nature Reviews Molecular Cell Biology 14: 141-152. (doi:10.1038/nrm3531).
McGinnis LK, Limback SD, Albertini DF (2013) Signaling modalities during oogenesis in mammals. In P M Wassarman, editor: Current Topics in Developmental Biology, Vol. 102,Academic Press, pp. 227-242. (ISBN: 978-0-12-416024-8).
Albertini, DF and Olsen,R (2013) Effects of fertility preservation on oocyte genomic integrity. In: Oocyte Biology in Fertility Preservation; Editor S S Kim; Chapter 3 Springer-Verlag, N.Y. pp 19-28.
Combelles, C.M.H. and Albertini, D.F. (2012).Over 40 years of mentoring, educating, and researching in the world of oocytes. An interview with professor Albertini. Int. J. Devel. Biol. 56:765-770. (doi: 10.1387/ijdb.120176cc)
Telfer E E and Albertini, DF (2012).The quest for human ovarian stem cells. Nature Medicine 18:353-354.
Limback, SD and Albertini, DF 2012 Imaging strategies for studying mammalian oogenesis. In "Oogenesis" Editors Coticchio, G., Albertini, D.F., and DeSantis, L. Ch.1. pp.3-18; Springer Publishing, N.Y.
Guglielmo, MC and Albertini, DF (2012) The structural basis for coordinating oogenesis and folliculogenesis. In: "Oogenesis" Editors Coticchio, G., Albertini, D.F., and DeSantis, L. Ch.7. pp.63-74; Springer Publishing, N.Y.
Albertini, DF (2012). Oocyte In Vitro Maturation: Formidable Obstacles on the Road to Fertility Preservation", In: Fertility Preservation, Eds. Seli, E., and Agarwal, A. Springer, London Ch. 10,pp121-128.
So IH, Hodges CA, Albertini DF, Hunt PA. (2011). Oocyte-specific differences in cell cycle control create an innate susceptibility to meiotic errors. Current Biology 21:651-657 DOI 10.1016/j.cub.2011.03.003
David F. Albertini, Ph.D.
3901 Rainbow Blvd. Mail Stop 3043
Kansas City, KS 66160