Microbiology, Molecular Genetics and Immunology
Ph.D., Chinese Center for Disease Control, Beijing, China, 1994.
Parvoviruses contain a small linear single-stranded DNA genome between 4.5-5.5 kb in length that has palindromic hairpin termini which serve as the origins of DNA replication. The viral genome is encapsidated within a non-enveloped, icosahedral virion, approximately 20 nm in diameter.
Parvoviruses that infect humans are 1) Human parvovirus B19 (B19V), which is the etiological agent of the fifth disease in children, several hematological disorders, including aplastic crisis and pure read cell aplasia, and hydrops fetalis in pregnant women; 2) Human bocavirus 1 (HBoV1) that causes lower respiratory tract infections in young children; and 3) Adeno-associated viruses (AAVs), which are non-pathogenic to humans.
My lab focuses on studying human parvoviruses B19V, HBoV1, and AAVs. The lab has chosen to investigate how these human parvoviruses utilize cellular DNA replication and DNA repair mechanisms for their DNA replication, how the viral genes are transcribed, processed, and translated to govern the production of progeny virions, and, for B19V and HBoV1, how the viral infections initiate, subvert the cellular defense mechanism, and eventually kill the infected cells. These fundamental studies will help understand the pathogenesis of human parvovirus infection, which will lead to identifying novel targets for the development of antiviral drugs to control viral infection and will also help to develop better parvoviral vectors for human gene therapy.
Current ongoing projects:
1. Molecular mechanism of B19V infection and replication in human erythroid progenitors.
2. Study of human parvovirus infection in human airway epithelia.
3. Development of chimeric recombinant AAV and HBoV1 vectors for gene delivery into human airways.
Wuxiang Guan, Fang Cheng, Qinfeng Huang, Steve Kleiboeker, and Jianming Qiu. Inclusion of the Central Exon of Parvovirus B19 Precursor mRNA is Determined by Multiple Splicing Enhancers both in the Exon and the Downstream Intron. Journal of Virology. 2011; 85:2463-8. PMC3067811
Wuxiang Guan, Qinfeng Huang, Fang Cheng, and Jianming Qiu. Internal Polyadenylation of the Parvovirus B19 Precursor mRNA is Regulated by Alternative Splicing. Journal of Biological Chemistry. 2011; 286: 24793-805. PMC3137055
Yong Luo, Sai Lou, Xuefeng Deng, Zhengwen Liu, Yi Li, Steve Kleiboeker, and Jianming Qiu. Parvovirus B19 infection of primary human erythroid progenitor cells triggers ATR-Chk1 signaling, which promotes B19 replication. Journal of Virology. 2011; 85:8046-8055. PMC3147961
Aaron Yun Chen, Steve Kleiboeker, and Jianming Qiu. Productive parvovirus B19 infection of primary human erythroid progenitor cells at hypoxia is regulated by STAT5A and MEK signaling but not HIFα. PLoS Pathogens. 2011; 7:e1002088. PMC3116823
Sai Lou, Baoyan Xu, Qinfeng Huang, Ning Zhi, Fang Cheng, Susan Wong, Kevin Brown, Eric Delwart, Zhengwen Liu, and Jianming Qiu. Molecular characterization of the newly identified human parvovirus 4 in the family Parvoviridae. Virology. 2012; 422:59-69. PMC3229647
Qinfeng Huang, Xuefeng Deng, Sonja M Best, Marshall E Bloom, Yi Li, and Jianming Qiu. Internal Polyadenylation of Parvoviral Precursor mRNA Limits Progeny Virus Production. Virology. 2012; 426:167-77. PMC3294060
Sai Lou S, Yong Luo, Fang Cheng, Qinfeng Huang, Weiran Shen, Steve Kleiboeker, John F. Tisdale, Zhengwen Liu, and Jianming Qiu. Human parvovirus B19 DNA replication induces a DNA damage response hat is dispensable for cell cycle arrest at phase G2/M. Journal of Virology. 2012; 86:10748-58. PMC3457271
Qinfeng Huang, Xuefeng Deng, Ziying Yan, Fang Cheng, Yong Luo, Weiran Shen, Diana C. M. Lei-Butters, Aaron Yun Chen, Yi Li, Liang Tang, Maria Söderlund-Venermo, John F. Engelhardt, and Jianming Qiu. Establishment of a reverse genetics system for studying human bocavirus in human airway epithelia. PLoS Pathogens. 2012; 8:e1002899. PMC3431310
Xuefeng Deng, Ziying Yan, Yong Luo, Jian Xu, Fang Cheng, Yi Li, John F. Engelhardt, and Jianming Qiu. In vitro modeling of human bocavirus 1 infection of polarized primary human airway epithelia. Journal of Virology. 2013; 87:4097-4102. PMC3624236
Yong Luo, Xuefeng Deng, Fang Cheng, Yi Li, and Jianming Qiu. SMC1-mediated intra-S phase arrest facilitates Bocavirus DNA replication. Journal of Virology. 2013; 87:4017-4032. PMC3624210
Ziying Yan, Nicholas W. Keiser, Yi Song, Xuefeng Deng, Fang Cheng, Jianming Qiu, and John F. Engelhardt: A Novel Chimeric Adeno-Associated Virus 2/Human Bocavirus 1 Parvovirus Vector Efficiently Transduces Human Airway Epithelia. Molecular Therapy. 2013; 21:2181-94. PMC3863803
Sunil Kumar Tewary, Haiyan Zhao, Weiran Shen, Jianming Qiu, and Liang Tang: Structure of the NS1 protein N-terminal origin-recognition/nickase domain from an autonomous parvovirus, the emerging human bocavirus. Journal of Virology. 2013; 87:11487-11493. PMC3807368
Yong Luo, Steve Kleiboeker, and Jianming Qiu: Human parvovirus B19 infection causes cell cycle arrest of human erythroid progenitors at late S phase that favors viral DNA replication. Journal of Virology. 2013; 87:12766-75. PMC3838147
Sunil Kumar Tewary, Haiyan Zhao, Xuefeng Deng, Jianming Qiu, and Liang Tang: The human parvovirus B19 non-structural protein 1 N-terminal domain specifically binds to the origin of replication in the viral DNA. Virology. 2014; 449:297-303. PMID: 24418564
Xuefeng Deng and Jianming Qiu: Human bocavirus 1 productively infects commercially-available primary human airway epithelium cultures. Journal of Virological Methods. 2014; 195:112-9. PMC3855471
Qinfeng Huang, Sonja M. Best, Marshall E. Bloom, and Jianming Qiu: Molecular Characterization of the Small Nonstructural Proteins of Parvovirus Aleutian Mink Disease Virus (AMDV) During Infection. Virology. 2014; 452:23-31. PMID: 24606679
Xi-Qun Shao, Yong-Jun Wen, Heng-Xing Ba, Xiu-Ting Zhang, Zhi-Gang Yue, Ke-Jian Wang, Chun-Yi Li, Jianming Qiu, Fu-He Yang: Discovery and Identification of a Novel Amdovirus Species that Infects Farmed Raccoon Dogs and Arctic Foxes. Emerging Infectious Disease. 2014; 20(12):2085-8. PMC4257837
Weiran Shen, Xuefeng Deng, Wei Zou, Fang Cheng , John F. Engelhardt, Ziying Yan, Jianming Qiu: Identification and Functional Analysis of Novel Nonstructural Proteins of Human Bocavirus 1. Journal of Virology. 2015; 89:10097-109. PMC4577888
Xuefeng Deng, Ziying Yan, Fang Cheng, John F. Engelhardt, Jianming Qiu: Replication of an Autonomous Human Parvovirus in Non-dividing Human Airway Epithelium Is Facilitated through the DNA Damage and Repair Pathways. PLOS Pathogens. 2016; 12(1):e1005399. PMC4713420
Wei Zou, Fang Cheng, Weiran Shen, John F. Engelhardt, Ziying Yan, Jianming Qiu: Nonstructural Protein NP1 of Human Bocavirus 1 Plays a Critical Role in the Expression of Viral Capsid Proteins. Journal of Virology. 2016; 90(9):4658-69. PMID: 26912614
Weiran Shen, Xuefeng Deng, Wei Zou, John F. Engelhardt, Ziying Yan, Jianming Qiu: Analysis of the Cis and Trans Requirements for DNA Replication at the Right End Hairpin of the Human Bocavirus 1 Genome. Journal of Virology. 2016; J Virol. 2016 Jun 22. pii: JVI.00708-16. [Epub ahead of print]
Jianming Qiu, Ph.D.
Office: 4004C Hixon
Lab: 4000 Hixon
Lab: (913) 588-4413