Jianming Qiu, Ph.D.
Director of Graduate Studies
Microbiology, Molecular Genetics and Immunology
Ph.D., Chinese Center for Disease Control, Beijing, China, 1994.
Parvoviruses contain a small linear single-stranded DNA genome between 4.5-5.5 kb in length that has palindromic hairpin termini which serve as the origins of DNA replication. The viral genome is encapsidated within a non-enveloped, icosahedral virion, approximately 20 nm in diameter.
Parvoviruses that infect humans are 1) Human parvovirus B19 (B19V), which is the etiological agent of the fifth disease in children, several hematological disorders, including aplastic crisis and pure read cell aplasia, and hydrops fetalis in pregnant women; 2) Human bocavirus 1 (HBoV1) that causes lower respiratory tract infections in young children; and 3) Adeno-associated viruses (AAVs), which are non-pathogenic to humans.
My lab focuses on studying human parvoviruses B19V, HBoV1, and AAVs. The lab has chosen to investigate how these human parvoviruses utilize cellular DNA replication and DNA repair mechanisms for their DNA replication, how the viral genes are transcribed, processed, and translated to govern the production of progeny virions, and, for B19V and HBoV1, how the viral infections initiate, subvert the cellular defense mechanism, and eventually kill the infected cells. These fundamental studies will help understand the pathogenesis of human parvovirus infection, which will lead to identifying novel targets for the development of antiviral drugs to control viral infection and will also help to develop better parvoviral vectors for human gene therapy.
Current ongoing projects:
1. Molecular mechanism of B19V infection and replication in human erythroid progenitors.
2. Study of human parvovirus infection in human airway epithelia.
3. Development of chimeric recombinant AAV and HBoV1 vectors for gene delivery into human airways.
Aaron Yun Chen, Elizabeth Yan Zhang, Wuxiang Guan, Fang Cheng, Steve Kleiboeker, Thomas M. Yankee, and Jianming Qiu. The small 11kDa non-structural protein of human parvovirus B19 plays a key role in inducing apoptosis during B19 virus infection of primary erythroid progenitor cells. Blood. 2010; 115:1070-1080. Featured in the “Inside Blood” section of this issue. PMC2817633
Aaron Yun Chen, Steve Kleiboeker, and Jianming Qiu. Productive parvovirus B19 infection of primary human erythroid progenitor cells at hypoxia is regulated by STAT5A and MEK signaling but not HIFα. PLoS Pathogens. 2011; 7:e1002088. PMC3116823
Qinfeng Huang, Xuefeng Deng, Ziying Yan, Fang Cheng, Yong Luo, Weiran Shen, Diana C. M. Lei-Butters, Aaron Yun Chen, Yi Li, Liang Tang, Maria Söderlund-Venermo, John F. Engelhardt, and Jianming Qiu. Establishment of a reverse genetics system for studying human bocavirus in human airway epithelia. PLoS Pathogens. 2012; 8:e1002899. PMC3431310
Ziying Yan, Nicholas W. Keiser, Yi Song, Xuefeng Deng, Fang Cheng, Jianming Qiu, and John F. Engelhardt: A novel chimeric adeno-Associated virus 2/human bocavirus 1 parvovirus vector efficiently transduces human airway epithelia. Molecular Therapy. 2013; 21:2181-2194. PMC3863803
Xuefeng Deng, Ziying Yan, Fang Cheng, John F. Engelhardt, Jianming Qiu: Replication of an autonomous human parvovirus in non-dividing human airway epithelium is facilitated through the DNA damage and repair pathways. PLOS Pathogens. 2016; 12:e1005399. PMC4713420
Peng Xu, Zhe Zhou, Min Xiong, Wei Zou, Xuefeng Deng, Safder S Ganaie, Steve Kleiboeker, Jianxin Peng, Kaiyu Liu, Shengqi Wang, Shui Qing Ye, and Jianming Qiu. Parvovirus B19 NS1 protein induces cell cycle arrest at G2-phase by activating the ATR-CDC25C-CDK1 pathway. PLOS Pathogens. 2017; 13:e1006266. PMC5354443
Zekun Wang, Weiran Shen, Fang Cheng, Xuefeng Deng, John F. Engelhardt, Ziying Yan, and Jianming Qiu. Parvovirus expresses a small noncoding RNA that plays an essential role in virus replication. Journal of Virology. 2017; 91:e02375-16. Featured in the “Spotlight” section of this issue. PMC5375684
Safder S Ganaie, Wei Zou, Peng Xu, Steve Kleiboeker, and Jianming Qiu. Phosphorylated STAT5 directly facilitates parvovirus B19 DNA replication in human erythroid progenitors through interaction with the MCM complex. PLOS Pathogens. 2017; 13:e1006370. PMC5426800
Xuefeng Deng, Wei Zou, Min Xiong, Zekun Wang, John F. Engelhardt, Shui Qing Ye, Ziying Yan, and Jianming Qiu. Human parvovirus infection of human airway epithelia induces pyroptotic cell death via inhibiting apoptosis. Journal of Virology. 2017; 91:e01533-17. PMC5709578
Zekun Wang, Fang Cheng, John F Engelhardt, Ziying Yan, Jianming Qiu. Development of a novel recombinant adeno-Associated virus production system using human bocavirus 1 helper genes. Molecular Therapy - Methods & Clinical Development. 2018; 11:40-51. PMC6205362
Peng Xu, Safder S. Ganaie, Xiaomei Wang, Zekun Wang, Steve Kleiboeker, Nancy C. Horton, Richard F. Heier, Marvin J. Meyers, John E. Tavis, and Jianming Qiu. Endonuclease Activity Inhibition of the NS1 Protein of Parvovirus B19 as a Novel Target for Antiviral Drug Development. Antimicrobial Agents and Chemotherapy. 2019; 63:e01879-18. PMC6395930