PI: Joe Lutkenhaus, Ph.D., University Distinguished Professor, Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center.
The Administrative Core is a key component of our Center and has major responsibilities in the administration of the overall program. The Core consists of the PI, the Director of the Pilot Project Program, the COBRE Administrator, the Accountant and both the Director of the Writing Core and an Editor. In the past 10 years the Core has been responsible for the oversight of 34 awards made on the advice of our External Advisory Committee (EAC). These awards have resulted in grant applications resulting in over $40 million in external grant awards. The COBRE Administrator is responsible for overall management and supervision of the daily business of the Center. In addition the Administrator compiles data, aides with grant applications of Center members, prepares reports, supplies administrative help for the pilot project program, organizes meetings and supervises the Accountant. The accountant tracks budgets for all projects and cores, aids in billing for the flow cytometry core, and processes budget expenditures for EAC members, meetings and seminars. The Director of the Writing Core provides technical editing services to Center members and writing workshops.
Core Director: Thomas Yankee, Pharm.D., Ph.D., Associate Professor, Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center
The KUMC flow cytometry core houses the Becton Dickinson Aria and the LSRII in the Department of Microbiology. Single cells are carried past 3 lasers in a fluid stream, fluorescence is measured via photomultiplier tubes which are placed inline with appropriate filters. Assistance is required with initial experiments to determine appropriate settings for data collection and interpretation as cells types vary in size, surface markers vary in density and therefore intensity, and investigators can select the combination of fluorochromes used; investigators will be able to work semi-independently in later appointments. Flow cytometry is especially powerful in that observations which can be made by microscopy will be made at an accelerated rate, in a quantitative manner, and on a single cell basis. Hundreds of corollary data points are collected to indicate cell size, granularity, and viability; when fluorescent dyes are added, activation of a cell can be measured by calcium flux or signal transduction by protein phosphorylation. Dyes can be used to quantitate proliferation or cell death, without radioactivity. Cells from mixed culture or tissue with specific properties can be separated and collected for additional study. A series of short topic based lectures is planned to introduce flow cytometry, flow applications, and software for data analysis.
Core Director: Martha Montello, Ph.D., Associate Professor, Department of History and Philosophy of Medicine, University of Kansas Medical Center
Writing Development Seminars
4 teaching workshops, 2 hours each, will be offered to the faculty in the COBRE training program for each year of the grant. The workshops will emphasize the basic principles and methods of clear and effective professional and academic writing, focusing on strategies necessary for publishing articles and writing grants. The workshops have been developed from Professor Montello's training at the University of Chicago's program for Academic and Professional Writing and honed through her two years of previous teaching with the K-30 Clinical Scholars Program.
Individual editing services
Two hours of manuscript editing and individual consultation services scheduled with the Writing Consult Center will be offered for the faculty members working on the grant, up to a total of 25 hours. With advance notification and scheduling, editing of individual manuscripts will have a turnaround time of 48 hours.
This grant was made possible by NIH Grant Number P20 RR016443 from the COBRE program of the National Center for Research Resources and NIH Grant Number P30 GM103326 from the COBRE Program of the National Institute of General Medical Sciences.