Department of Pharmacology, Toxicology & Therapeutics
Ph.D., Medical College of Wisconsin, 2001
Postdoctoral, St. Jude Children's Research Hospital, 2001-2007
The overall research interest of the laboratory is to understand the role of ATP binding cassette (ABC) transporters in Biology and Medicine. These proteins, found in all species, use the energy of ATP hydrolysis to translocate specific substrates across cell membranes. Most of these transporters play important roles in normal biology and in the therapeutic response to medications.
Our current studies focus on 1) Identifying the physiological function, regulation and clinical significance of ABC transporters and 2) Understanding the significance of protein-protein interactions to the intracellular trafficking and functional activity of these multi domain transport machineries. We explore these questions through molecular and cellular biology, protein biochemistry and genetics. We complement our cell culture studies with whole animal studies to put our finding at the cellular level in the context of the whole animal. We expect that these studies will not only provide insight into the cellular role of these transporters but also help us translate this knowledge into new diagnostic and treatment strategies to optimize therapy.
Krishnamurthy P, Ross DD, Nakanishi T, Zhou S, Mercer KE, Sarkadi B, Sorrentino BP and Schuetz JD. (2004) The stem cell marker Bcrp/ABCG2 enhances hypoxic cell survival through interactions with heme. J Biol Chem. 279:24218-24225.
Krishnamurthy P and Schuetz JD. (2006) Role of ABCG2/BCRP in Biology and Medicine. Annu Rev Pharmacol. 46:381-410.
Krishnamurthy P, Du G, Fukuda Y, Daxi S, Sampath J, Mercer KE, Wang J, Sosa-pineda B, Murti G and Schuetz JD. (2006) Identification of a mitochondrial heme importer that regulates erythroid gene expression and heme biosynthesis. Nature. 443:586-589.
Li C, Krishnamurthy P, Penmatsa H, Mars KL, Wang X, Zaccolo M, Jalink K, Li M, Nelson DJ, Schuetz JD and Naren AP. (2007) Spatiotemporal coupling of cAMP Transporter to CFTR Chloride Channel Function in the Gut Epithelia. Cell. 131:940-951.
Polireddy K, Chavan H, Abdulkarim BA and Krishnamurthy P. (2011) Functional significance of the ATP-binding cassette transporter B6 in Hepatocellular carcinoma. Molecular Oncology. 5:410-423.
Polireddy K, Khan MM, Chavan H, Young S, Waller A, Perez D, Chavez S, Strouse JJ, Haynes MK, Bologa CG, Opera TI, Tegos GP, Sklar LA and Krishnamurthy P. (2012) A novel HTS flow cytometric assay reveals functional modulators of the ATP binding cassette transporter ABCB6. PLoS One. 7:e4005.
Chavan H, Krishnamurthy P. (2012) Polycyclic aromatic hydrocarbons (PAHs) mediate transcriptional activation of the ATP binding cassette transporter ABCB6 gene via the Aryl Hydrocarbon receptor. J Biol Chem. 287:32054-32068.
Chavan H, Khan MM, Tegos GP and Krishnamurthy P. (2013) Efficient purification and reconstitution of ABCB6 for functional and structural studies. J Biol Chem. 288:22658-22669.
Partha Kasturi, Ph.D.