Melissa A. Larson, Ph.D.

Research Assistant Professor
Technical Director, Transgenic and Gene-Targeting Institutional Facility
Department of Molecular and Integrative Physiology

Ph.D., University of Missouri, 2000


mlarson@kumc.edu

Dr. Larson serves as the Technical Director of the Transgenic and Gene-Targeting Facility, a shared, core, research support facility providing a centralized service for the production of transgenic and gene-targeted mice for the investigators of KUMC and the surrounding Kansas City research community. The facility provides services that include the generation of transgenic mice by pronuclear microinjection, generation of chimeric mice by blastocyst injection, targeting of embryonic stem cells, sperm and embryo cryopreservation and genotyping of mice. The facility provides consultation on new projects and training and demonstration in microinjection, embryo transfer surgeries and ES cell culture. The lab is also investigating new technologies that improve the production and maintenance of gene-modified mice, as well as introducing new services and technologies to our users.  In addition, Dr. Larson's laboratory has conducted experiments to determine whether a novel recombinase, Dre, functions in mice. Demonstration of its action has provided another tool to manipulate the mouse genome in vivo.

Selected Publications

Home P, Saha B, Ray S, Dutta D, Gunewardena S, Yoo B, Pal A, Vivian JL, Larson M, Petroff M, Gallagher PG, Schulz VP, White KL, Golos TG, Behr B, Paul S (2012) Altered subcellular localization of transcription factor TEAD4 regulates first mammalian cell lineage commitment. Proc Natl Acad Sci USA 109(19):7362-7.

Dutta D, Ray S, Home P, Larson M, Wolfe MW, Paul S (2011) Self renewal vs. lineage commitment of embryonic stem cells: Protein Kinase C signaling shifts the balance. Stem Cells 29(4):618-628. 

Kumar TR, Larson M, Wang H, McDermott J, Bronshteyn I (2009) Transgenic Mouse Technology: Principles and Methods. In: "Molecular Endocrinology: A comprehensive guide to current methodologies" Editors: Park-Sarge O-K & Curry TE, Humana Press New Jersey 590:335-62.    

Home P, Ray S, Dutta D, Bronshteyn I, Larson M, Paul S (2009) GATA3 is selectively expressed in the trophectoderm of peri-implantation embryo and directly regulates Cdx2 gene expression. J Biol Chem 284(42):28729-28737.

Dufner-Beattie J, Weaver BP, Geiser J, Bilgen M, Larson M, Xu W, Andrews GK (2007) The mouse acrodermatitis enteropathica gene Slc39a4 (Zip4) is essential for early development and heterozygosity causes hypersensitivity to zinc deficiency. Hum Mol Genet 16(12):1391-9.

Last modified: Dec 28, 2012

Contact

Melissa A. Larson, Ph.D.
Research Assistant Professor
Technical Director, Transgenic and Gene-Targeting Institutional Facility

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