Jay L. Vivian, Ph.D.

Scientific Director, KUMC Transgenic Facility
Research Associate Professor
Department of Pathology and Laboratory Medicine

Ph.D., University of Texas-Houston, 1999
Postdoctoral, University of North Carolina at Chapel Hill, 2000-2004


jvivian@kumc.edu

My research uses embryonic and induced pluripotent stem cells as genetic and developmental models to study signaling during embryonic development and the regulation of pluripotency.  My group is interested in understanding the signaling pathways and genetic hierarchies that regulate gene expression and stem cell self-renewal in embryonic stem cells.  A major project seeks to understand the function of TGF-beta-related signaling pathways in stem cell self-renewal.  These efforts have uncovered important and previously uncharacterized roles for the Nodal and BMP signaling pathways in the dynamic heterogeneity that exists in pluripotent cells.  A major effort in our lab focuses on understanding heterogeneity in undifferentiated cells: how heterogeneity arises and how it may be regulated.  This basic research question has a major impact on understanding the complex phenotype of pluripotency and stem cell self-renewal.

A major focus of my group also involves the use of pluripotent stem cells as models of human disease.  Our work aims to develop cellular therapies for spinal cord injury using human induced pluripotent stem (iPS) cells differentiated to specific neural lineages.  This work makes use of novel engineering strategies for introducing reporters into human iPS cells to monitor directed neural differentiation, and for use in high-throughput screens.

My group makes substantial use of mouse embryonic stem cells for genetic engineering to generate transgenic and mutant mice, for the development of animal models of congenital diseases mapped by human genetics studies. I have a sustained interest in developing novel strategies to generate mutant and transgenic animals to support these studies. These efforts leverage our knowledge of both stem cell and developmental biology.  

Selected Publications

Vivian JL, Chen Y, Yee D, Schneider E, Magnuson T. (2002) An allelic series of mutations in Smad2 and Smad4 identified in a genotype-based screen of N-ethyl-N-nitrosourea-mutagenized mouse embryonic stem cells. Proc Natl Acad Sci U S A. 99, 15542-7.

Chen Y, Vivian JL, Magnuson T. (2003) Gene-based chemical mutagenesis in mouse embryonic stem cells. Methods Enzymol. 365, 406-15.

Vivian JL, Chen Y, Magnuson, T. (2004) Gene-based screens of chemically mutagenized mouse embryonic stem cells.  In: Handbook of Stem Cells. Lanza RP, et al (Eds), Academic Press.  

Kirn-Safran CB, Oristian DS, Focht RJ, Parker SG, Vivian JL, Carson DD. (2007) Global growth deficiencies in mice lacking the ribosomal protein HIP/RPL29. Dev Dyn. 236, 447-60.

Dutta D, Ray S, Vivian JL, Paul S. (2008) Activation of the VEGFR1 chromatin domain: an angiogenic signal-ETS1/HIF-2alpha regulatory axis. J Biol Chem. 283, 25404-13.

Galvin KE, Travis ED, Yee D, Magnuson T, Vivian JL. (2010) Nodal signaling regulates the bone morphogenic protein pluripotency pathway in mouse embryonic stem cells. J Biol Chem. 285, 19747-56.

Feng Y, Yang Y, Ortega MM, Copeland JN, Zhang M, Jacob JB, Fields TA, Vivian JL, Fields PE. (2010) Early mammalian erythropoiesis requires the Dot1L methyltransferase. Blood. 116, 4483-91.

Copeland JN, Feng Y, Neradugomma NK, Fields PE, Vivian JL. (2011). Notch signaling regulates remodeling and vessel diameter in the extraembryonic yolk sac. BMC Dev  Biol. 11, 12.

Burgess-Galvin, K.E. and Vivian, J.L. (2011). Transforming Growth Factor-beta superfamily in mouse embryonic stem cell self-renewal. Vitamins and Hormones 87: Stem Cell Regulators. 87, 341-65.

Home, P, Biswarup Saha, B., Ray, S., Debasree Dutta, D., Gunewardena, S., Yoo, B., Vivian, J.L., Larson, M., Petroff, M. , Gallagher, P.G., Schulz, V., White, K.L., Thaddeus G. Golos, T.G. , Behr, B., Paul, S. (2012). Altered Subcellular Localization of Transcription Factor TEAD4 Regulates First Mammalian Cell Lineage Commitment. PNAS 109, 7362-7.

Burgess-Galvin, K.E., Travis, E.D., Pierson, K.E., and Vivian, J.L. (2013).  TGF-beta-related signaling in embryonic stem cell maintenance: self-renewal as a dynamic and regulated equilibrium. Stem Cells 31, 48-58.

Last modified: Sep 24, 2013

Jay L. Vivian, Ph.D.

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Jay L. Vivian, Ph.D.
Scientific Director, KUMC Transgenic Facility
Research Associate Professor

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