Fernando Pierucci-Alves, DVM

Research Assistant Professor
Department of Anatomy & Physiology, Kansas State University

DVM, Federal University of Uberlandia, Brazil, 1999
Postdoctoral, Kansas State University, 2004-2008


Sperm cells mature and acquire fertilizing capacity while transiting through the lumen of the male excurrent system, which is composed by the efferent ducts, epididymis and vas deferens. This ductal system is lined in its entirety by epithelial cells carrying out an array of secretory and absorptive mechanisms. These epithelial activities are required for successful spermatozoa maturation and fertility. Our research program is dedicated to understanding how growth factors, specifically transforming growth factor beta (TGFβ) regulate epithelial and sperm cell biology. By enhancing our knowledge of how this signaling pathway participates in the physiology of this organ system, we expect to form the basis for future diagnostics and treatment of infertility cases that remain primarily undiagnosed.

Selected Publications

Pierucci-Alves F, Yi S, Schultz BD. Transforming Growth Factor Beta 1 Induces Tight Junction Disruptions and Loss of Transepithelial Resistance Across Porcine Vas Deferens Epithelial Cells. Biol Reprod 86: 36, 1-8, 2012.

Pierucci-Alves F, Akoyev V, Stewart JC 3rd, Wang LH, Janardhan K, Schultz BD. Swine models of cystic fibrosis reveal male reproductive tract phenotype at birth. Biol Reprod 85:442-51, 2011.

Ferrer MS, Lutjemeier BJ, Koopman T, Pierucci-Alves F, Weiss ML. Xenogeneic transplantation of equine testicular cells into seminiferous tubules of immunocompetent rats. Theriogenology 75:1258-64, 2011.

Pierucci-Alves F, Duncan CL, Lillich JD, Schultz BD. Porcine vas deferens luminal pH is acutely increased by systemic xylazine administration. Biol Reprod 82: 132-135, 2010.

Pierucci-Alves F, Duncan CL, Schultz BD. Testosterone upregulates anion secretion across porcine vas deferens epithelia in vitro. Biol Reprod 81: 628-635, 2009.

Pierucci-Alves F, Schultz BD. Bradykinin-stimulated cyclooxygenase activity stimulates vas deferens epithelial anion secretion in vitro in swine and humans. Biol Reprod 79: 501-509, 2008.

Carlin RW, Sedlacek RL, Quesnell R, Pierucci-Alves F, Grieger DM, Schultz BD. PVD9902, a porcine vas deferens epithelial cell line that exhibits neurotransmitter-stimulated anion secretion and expresses numerous HCO3--transporters. Am J Physiol Cell Physiol 290: 1560-1571, 2006.

Schmahl J, Yao HH, Pierucci-Alves F, Capel B. Colocalization of WT1 and cell proliferation reveals conserved mechanisms in temperature-dependent sex determination. Genesis 35 (4): 193-201, 2003.

Pierucci-Alves F, Clark AM, Russell LD. A Developmental study of the Desert Hedgehog-null mouse testis. Biol Reprod 65: 1392-1402, 2001.


Last modified: Jul 28, 2014


Fernando Pierucci-Alves, DVM
Research Assistant Professor