Department of Obstetrics and Gynecology
Ph.D., University of Illinois, Urbana-Champaign, 2003
Postdoctoral, Stanford University, 2003-2010
The primary focus of our laboratory is the study of the transcriptional mechanisms activated in response to reduced cellular oxygen, or hypoxia. A significant proportion of hypoxic gene expression is mediated by the Hypoxia Inducible Factors (HIFs), transcription factors that induce the expression of genes important for anaerobic metabolism, blood vessel recruitment, cell motility, and stem cell maintenance. Of particular interest is the hypoxic expression of several histone demethylase genes by the HIFs. Since histone demethylases affect gene expression by modifying the chromatin of target genes, hypoxic regulation of this phenomenon creates an intriguing link between cellular microenvironment, HIF activation, and downstream cascades of gene expression that could prolong the initial cellular response to hypoxia. We are currently studying the functional consequences of hypoxic histone demethylase expression in the context of normal cell biology and in disease states ranging from cancer to intrauterine growth restriction.
Krieg SA, Fan X, Hong Y, Sang QX, Giaccia A, Westphal LM, Lathi RB, Krieg AJ, Nayak NR. Global alternation in gene expression profiles of deciduas from women with idiopathic recurrent pregnancy loss. Mol Hum Reprod. 2012 Sep:18(9):442-50.
Krieg AJ, Rankin EB, Chan D, Razorenova O, Fernandez S, Giaccia AJ. Regulation of the histone demethylase JMJD1A by hypoxia-inducible factor 1 alpha enhances hypoxic gene expression and tumor growth. Mol Cell Biol. 2010 Jan;30(1):344-53.
Basak S, Jacobs SB, Krieg AJ, Pathak N, Zeng Q, Kaldis P, Giaccia AJ, Attardi LD. The metastasis-associated gene Prl-3 is a p53 target involved in cell-cycleregulation. Mol Cell. 2008 May 9;30(3):303-14.
Sutphin PD, Chan DA, Li JM, Turcotte S, Krieg AJ, Giaccia AJ. Targeting the loss of the von Hippel-Lindau tumor suppressor gene in renal cell carcinomacells. Cancer Res. 2007 Jun 15;67(12):5896-905.
Krieg AJ, Hammond EM, Giaccia AJ. Functional analysis of p53 binding under differential stresses. Mol Cell Biol. 2006 Oct;26(19):7030-45.
Hammond EM, Mandell DJ, Salim A, Krieg AJ, Johnson TM, Shirazi HA, Attardi LD, Giaccia AJ. Genome-wide analysis of p53 under hypoxic conditions. Mol Cell Biol. 2006 May;26(9):3492-504.
Krieg AJ, Krieg SA, Ahn BS, Shapiro DJ. Interplay between estrogen response element sequence and ligands controls in vivo binding of estrogen receptor to regulated genes. J Biol Chem. 2004 Feb 6;279(6):5025-34.
Adam J. Krieg, Ph.D.