Kurt E. Ebner, Ph.D.

Professor Emeritus
Department of Biochemistry and Molecular Biology

University of Illinois, Urbana, IL, Ph.D., 1960
University of Reading, England (Postdoctoral Fellow)
University of Minnesota (Postdoctoral Fellow, Instructor)
Oklahoma State University (Assistant Professor, Associate Professor, Regents Professor)
University of Reading, England (Postdoctoral Fellow)
University of Minnesota (Postdoctoral Fellow, Instructor)
Oklahoma State University (Assistant Professor, Associate Professor, Regents Professor)
University of Reading, England (Postdoctoral Fellow)
University of Minnesota (Postdoctoral Fellow, Instructor)
Oklahoma State University (Assistant Professor, Associate Professor, Regents Professor)
University of Reading, England (Postdoctoral Fellow)
University of Minnesota (Postdoctoral Fellow, Instructor)
Oklahoma State University (Assistant Professor, Associate Professor, Regents Professor)


kebner@kumc.edu

Major Research Interests

Recent research efforts focus on determining how the protein hormone prolactin interacts with its receptor, which is the first step in understanding how this class of hormones functions. We have expressed the extracellular domain of the rat liver prolactin receptor in the vaccinia virus expression system and showed that the purified extracellular domain forms a complex with prolactin of the type: (extracellular domain)2 (prolactin). Studies using site-directed mutagenesis will be used to determine the amino acids necessary to form this complex and how the complex initiates signal transduction in the cell.

Another study has focused on the interaction between a sense peptide found in prolactin and its corresponding anti-sense peptide derived from the minus strand of the prolactin DNA. These peptides form a complex in solution and the antisense peptide forms antibodies which recognize the prolactin receptor. Future studies using microcalorimetry will focus on determining how these peptides interact in solution.

Representative Publications

Hooper, K.P., Padmanabhan, R. and Ebner, K.E. (1993) Expression of the extracellular domain of the rat liver prolactin recptor and its interaction with ovine prolactin. J. Biol. Chem. 268, 22347-22352.

Bajpai, A., Hooper, K.P. and Ebner, K.E. (1991) Interactions of antisense peptides with ovine prolactin. Biochem. Biophys. Res. Commun. 180, 1312-1317.

Kalluri, R., Gunwar, S., Reeders, S.T., Morrison, K.C, M., Ebner, K.E.,  Noelken, M. and Hudson, B.G. (1991) Goodpasture syndrome: localization of the epitope for the autoantibodies to the carboxyl terminal region of the 3 (IV) chain of basement membrane collagen. J. Biol. Chem. 266, 24018-24024.

Nair, M.K., Ebner, K.E. and Cook J.D. (1990) Isolation and characterization of a transferrin binding protein from rat plasma. Biochim. Biophys. Acta 1035, 306-312.

Varma, S., Kwok, S. and Ebner, K.E. (1989) Cloning and nucleotide sequence of ovine prolactin cDNA. Gene 77, 349-359.

Bajpai, A., Andrews, G.K. and Ebner, K.E. (1989) Induction of c-fos mRNA in rat lymphoma Nb-2 cells. Biochem. Biophys. Res. Commun. 165 1359-1363.

Pollock, H.G., Hamilton, J.W., Rouse, J.B., Ebner, K.E. and Rawitch, A.B. (1988) Isolation of peptide hormones from the pancreas of the bullfrog (Rana catesbeiana). J. Biol. Chem. 263, 9746-975l.

Taylor, T.C., Thompson, D.O., Ebner, K.E., Kimmel, J.R. and Rawitch, A.B. (1988) An immunochemical study of aylan pancreatic polypeptide: the nature of the principal epitope. Mol. Immunol. 25, 961-973.

Varma S. and Ebner, K.E. (1988) The effect of cyclosporin A on the growth and prolactin binding to Nb-2 rat lymphoma cells. Biochem. Biophys. Res. Commun. 156, 233-239.

Andrews, G.K., Varma, S. and Ebner, K.E. (1987) Regulation of expression of c-fos and c-myc in rat lymphoma cells. Biochem. Biophys. Acta 909, 231-236.


Last modified: Jul 29, 2013

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Kurt E. Ebner, Ph.D.
Professor Emeritus

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