Robert C. De Lisle, PhD
Ph.D.: 1984, Case Western Reserve University, Cleveland, OH
Postdoctoral: University of California, San Francisco
The focus of my research is investigation of pathobiology of cystic fibrosis (CF) in the gastrointestinal (GI) system.
CF is one of the most common life-shortening genetic diseases in Caucasians and is caused by mutations in the CFTR gene. CFTR is a cAMP-regulated chloride channel expressed in many epithelial cells. In the absence of functional CFTR, exocrine secretions are poorly-hydrated and viscous mucus covers affected epithelial surfaces. In the GI system accumulation of mucus can lead to obstruction of the lumen. Accumulated mucus also provides a niche for abnormal bacterial colonization and overgrowth. In the healthy gut, gastrointestinal motility is an important control for bacterial load, especially in the proximal small intestine. GI motility has been reported to be impaired in about ½ of CF patients, and this may contribute to bacterial overgrowth. These effects of CF interfere with proper nutrition, are detrimental to overall health, and lower the quality of life in CF patients.
Using the Cftr knockout mouse (Cftr tm1UNC) model of CF we are investigating the inter-related roles of excessive mucus, abnormal bacterial growth, and impaired motility in the pathophysiology of CF in the intestine. Pharmacological and dietary approaches are being used to determine the mechanisms of intestinal dysfunction in CF. Recent work from our lab shows significant changes in expression of genes whose products control prostaglanind levels, and these changes are implicated in the CF pathophysiology. The long term goal is to provide new therapeutic interventions to improve intestinal function in CF. This work includes in vivo and in vitro studies; morphological and immunocytochemical techniques; and measurement of mRNA and protein levels for various inflammatory markers and signaling molecules.
Current Postdoctoral Fellows
Former trainees (Postdoc & Graduate Students)
- Oxana Norkina, M.D., postdoctoral fellow Univ Mass School of Medicine, Worcester, MA
- Igor Boulatnikov, PhD., Senior Scientist Univ Kansas School of Medicine, Kansas City, KS
- Subramanian Venkateswaran, PhD; Department of Biochemistry, Annamalai University, Tamil Nadu, India
- Abdulbaki (Baki) Agbas, PhD; Research Assistant Professor, University of Kansas, Lawrence, KS; email@example.com
- Simran Kaur, Ph.D., homemaker; firstname.lastname@example.org
- Chanderdeep Tandon, Ph.D., Instructor, Jaypee University of Information Technology, India; email@example.com
Click here for Complete List of Publications from PubMed
- De Lisle,R.C., L.Meldi, M.Flynn, and K.Jansson. 2008. Altered eicosanoid metabolism in the cystic fibrosis mouse small intestine. J Pediatr Gastroenterol Nutr 47:406-416.
De Lisle,R.C., L.Meldi, E.Roach, M.Flynn, and R.Sewell. 2009. Mast cells and gastrointestinal dysmotility in the cystic fibrosis mouse. PLos ONE 4:e4283
- De Lisle,R.C., E.Roach, and K.Jansson. 2007. Effects of laxative and N-acetylcysteine on mucus accumulation, bacterial load, transit, and inflammation in the cystic fibrosis mouse small intestine. Am J Physiol Gastrointest Liver Physiol 293:G577-G584
- De Lisle,R.C., E.A.Roach, and O.Norkina. 2006. Eradication of small intestinal bacterial overgrowth in the cystic fibrosis mouse reduces mucus accumulation. J Pediatr Gastroenterol Nutr 42:46-52.
- De Lisle,R.C., W.Xu, B.A.Roe, and D.Ziemer. 2008. Effects of Muclin (Dmbt1) Deficiency on the Gastrointestinal System. Am J Physiol Gastrointest Liver Physiol 294:G717-G727
- Magenheimer,B.S., P.L.St John, K.S.Isom, D.R.Abrahamson, R.C.De Lisle, D.P.Wallace, R.L.Maser, J.J.Grantham, and J.P.Calvet. 2006. Early embryonic renal tubules of wild-type and polycystic kidney disease kidneys respond to cAMP stimulation with cystic fibrosis transmembrane conductance regulator/Na(+),K(+),2Cl(-) Co-transporter-dependent cystic dilation. J.Am.Soc.Nephrol. 17:3424-3437.
- Norkina,O. and R.C.De Lisle. 2005. Potential genetic modifiers of the cystic fibrosis intestinal inflammatory phenotype on mouse chromosomes 1, 9, and 10. BMC.Genet. 6:29
- Norkina,O., R.Graf, P.Appenzeller, and R.C.De Lisle. 2006. Caerulein-Induced Acute Pancreatitis in Mice that Constitutively Overexpress Reg/PAP Genes. BMC.Gastroenterol. 6:16
Bob De Lisle (PI), Racquel Sewell (Res.Asst.), Lauren Meldi (Res.Asst.), Maureen Flynn (Res.Asst.)
Dec 04, 2012