TMD Change[1]

Variant

Assembly

Y282D Reversion[2]

Protein Stability

Chemical Modification

Solvent Exposure

Compared to WT

Monoclonal Exposure

UV Difference

Y7

W/Wless[3]

M42

I

X

L62

W/Wless[3]

I64

1

V66

1

L71

2

L73

3

H74

2

R
/Wless[3]

X

S77

2

L[4]

ANS more apolar
KI: Less quenching/solvent exposure with IPTG change

Bigger >300 nm

Q78

1

V80

1

A81

V[5]

C107 IPTG sensitivity lost

Very small diff

I83

1

K84

1,2

E
R
A
L

 X[6]
X 

High[7]
Very high

WT6
WT
Decreased
Decreased

 All bigger[8] peaks

All bigger peaks

D88

1

L90

1

S93

1

V94

1

V95

V96

1

S97

1

M98

1

I

X

E100

1

W/Wless[3]

N113

1