Post Graduate Institute of Medical Education & Research, 2002.
5031 Wahl West, MS 3043
3901 Rainbow Blvd.
Kansas City, KS 66160
(913) 588-5027
ndhillon@kumc.edu
Research
My research involves understanding the interplay of macrophages, cytokines and chemokines in lung infections associated with HIV-infection. One of my primary research efforts is aimed at developing strategies using antisense (AS) DNA of host factors (cytokines) for abrogation of virus replication. Using this approach I also plan to examine the molecular mechanism(s) by which cytokines regulate virus replication. Our research showed that administration of liposomes containing AS DNA against IL-4, a host factor which causes enhancement of virus replication, to SHIV-infected rhesus macaques caused reduction of virus replication in the lungs, spleen, and lymph nodes and in the blood of the infected animals. I am now specifically focusing on the use of nanoparticles to deliver AS IL-4 DNA to chronically infected pneumonic macaques in an attempt to curtail replication of the virus in lungs, thereby reducing the disease pathogenesis. Such an approach using particles in the nanometer range is advantageous in that it is safe, cost effective, do not generate immune responses and can effectively deliver therapeutic genes over longer range of time. This work will further provide an opportunity to evaluate the efficacy of AS DNA containing nanoparticles for therapy of other immune-mediated diseases.
Peer Reviewed Publications:
Navneet K. Dhillon, Rachel Williams, Shannon Callen, Chris Zein, Opendra Narayan and Shilpa Buch.2007, Roles of MCP-1 in Development of HIV-Dementia. Frontiers in Biosciences (submitted)
Fuwang Peng, Navneet K. Dhillon, Sirosh Bokhari, Xuhui Zhu, Hicham. H. Baydoun, Shilpa Buch. 2007, Platelet- Shannon Callen, Derived Growth Factor Protects Neurons against Gp120-Mediated Toxicity. J Neurovirology (submitted).
Navneet K. Dhillon, Fuwang Peng, Sirosh Bokhari, Shannon Callen, , Sun-Hye Shin*, Xuhui Zhu†, Kee-Jun Kim*, Shilpa Buch.2007,Cocaine-mediated Alteration in Tight Junction Protein Expression and Modulation of CCL2/CCR2 axis across the Blood Brain Barrier: Implications for HIV-Dementia. J Neuroimmuno Pharmacology (accepted).
Navneet K. Dhillon, Fuwang Peng, Richard R. Ransohoff, Shilpa Buch. 2007, PDGF Synergistically Enhances IFN-g Induced Expression of CXCL10 in Blood-Derived Macrophages: Implications for HIV-Dementia. J. Immunology 2007 Sep 1; 179(5):2722-30.
Navneet K. Dhillon, Rachel Williams, Fuwang Peng, Yi-jou Tsai, Sukhbir Dhillon, Brandon Nicolay, Milind Gadgil and Shilpa J. Buch. 2007, Cocaine-mediated Enhancement of Virus Replication in Macrophages: Implications for HIV-Dementia. J Neurovirology (accepted).
Navneet K. Dhillon, Milind Gadgil, Andy Rahardja, Himanshu Kenjale, Alex Sidelnik, Duncan Renfrow, Amanda†, Sukhbir Dhillon, Anil Kumar and Shilpa J. Buch†. 2007. Cocaine: A Catalyst for Human Immunodeficiency Virus-Associated Dementia. Am. J. Infectious Diseases. (accepted)
Navneet K. Dhillon, D Pinson, S Dhillon, O Tawfik, M Danley, M Davis, O Nemon, M Mayo, A Kumar, Y Tsai, A Kumar, and S Buch. 2007. Bleomycin Treatment causes Enhancement of Virus Replication in the Lungs of SHIV- infected Macaques. Am J Physiol Lung Cell Mol Physiol. May; 292(5):L1233-40.
Navneet K. Dhillon*,Yongjun Sui*, David Pinson, Shanping Li, Sukhbir Dhillon, Ossama Tawfik, Shannon Callen, Olga Nemon, Opendra Narayan, Shilpa Buch (*co first authors ).2007. Up-regulation of PDGF and its Receptor Expression in Simian-Human Immunodeficiency Virus-Associated Pneumonia in Macaques. AIDS. Jan 30; 21(3):307-316.
Abhik Datta, Uma Sinha-Datta, Navneet K. Dhillon, Shilpa Buch and Christophe Nicot. 2006. HTLV-I and innate immunity: Interference with TLR4 signaling by p30 modulates the release of pro-and anti-inflammatory cytokines from human macrophages. J Biol Chem. Aug 18;281(33):23414-24
Navneet K. Dhillon, Sukhbir Dhillon, Yahia Chebloune, David Pinson Francois Villinger and Shilpa Buch. 2006. Therapy of SHIV Infected Macaques with Liposomes Delivering Antisense IL-4 DNA. AIDS. May 12;20(8):1125-30
Yongjun Sui, Lisa Stehno-Bittel, Shanping Li, Rajprasad Loganathan, Navneet K. Dhillon, David Pinson, Avindra Nath, Dennis Kolson, Opendra Narayan, Shilpa Buch.2006. CXCL10-induced Cell Death in Neurons: Role of Calcium Dysregulation. Eur J Neurosci. Feb;23(4):957-64.
Navneet K. Dhillon, Yongjun Sui, Raghava Potula, Sukhbir Dhillon, Istvan Adany, Zhuang Li, Francois Villinger, David Pinson, Opendra Narayan, and Shilpa Buch. 2005. Inhibition of Pathogenic SHIV Replication in Macaques Treated With Antisense DNA of Interleukin-4. Blood. April; 105(8):3094-3099.
Shilpa Buch, Yongjun Sui, Navneet Dhillon, Raghava Potula, Christopher Zien, David Pinson, Shanping Li, Sukhbir Dhillon,Brandon Nicolay, Alex Sidelnik, Cicy Li, Tara Villinger, Kunal Bisarriya and Opendra Narayan.2004. Cytokines and Chemokines as perpetrators of Simian Human Immunodeficiency Virus-Encephalitis. J Neuroimmunology Dec;157(1-2):71-80. Review.
Raghava Potula, Navneet Dhillon, Yongjun Sui, Christopher Zien, Keiko Funa, David Pinson, Opendra Narayan and Shilpa Buch.2004 Association of Platelet-derived Growth Factor-B chain with Simian Human Immunodeficiency Virus Encephalitis. American J Pathology 165(3)
Yongjun Sui, Raghava Potula, Navneet Dhillon , David Pinson, Shanping Li, Avindra Nath, Carol Anderson, Dennis Kolson, Opendra Narayan, and Shilpa Buch. 2004. Neuronal Apoptosis is mediated by CXCL 10 over-expression in Simian Human Immunodeficiency Virus-Encephalitis. American J Pathology 164(5):1557-1566
Buch S, Y Sui, R Potula, D Pinson, I Adany, Z Li, S Li, Navneet Dhillon, E major and O Narayan .2003. Role of interleukin-4 and monocyte chemoattractant protein-1 in the neuropathogenesis of X4-SHIV infection in macaques. J. Neurovirology 10 suppl 1:118-24
Navneet K. Dhillon, S.Sharma, G.K.Khuller. 2003. Signaling through protein kinases and transcriptional regulators in Candida albicans. Crit. Rev.Microbiol.29: 259-75.
Navneet K. Dhillon, S.Sharma, G.K.Khuller. 2003. Biochemical characterization of Ca2+/calmodulin dependent protein kinase from Candida albicans. Mol.cell.Biochem. 252: 183-191.
Navneet K. Dhillon, S.Sharma, G.K.Khuller. 2003. Influence of W-7, a calmodulin antagonist on phospholipid biosynthesis in Candida albicans. Lett Appl Microbiol. 36(6): 382-6.
Navneet Kaur, Jyotdeep Kaur and Akhtar Mahmood. 2002. Studies on Harmaline interactions with brush border sucrase in rat intestine. Ind. J Biochem. Biophys. 39:119-123.
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