Methodologies

Currently the following methodologies workflows are implemented:

In gel and in solution trypsin digestion
Protein identification from 1D and 2D PAGE by peptide mass fingerprint
Mass spectrometry protein/peptide mass measurement, including protein desalting using reverse phase chromatography.
Peptide sequencing and “bottom-up” protein identification.
Multidimensional Protein identification technology for the analysis of complex protein mixtures (MudPIT)
Protein-protein interactions (experimental protocol).
Protein dynamics by a combination of deuterium exchange-mass spectrometry.
Protein quantification by mass spectrometry: iCAT, SILAC, free label, AAUA peptides,...

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