Methodologies

Currently the following methodologies workflows are implemented:

  • In gel and in solution trypsin digestion
  • Mass spectrometry protein/peptide mass measurement, including protein desalting using reverse phase chromatography.
  • Peptide sequencing and "bottom-up" protein identification.
  • Multidimensional Protein identification technology for the analysis of complex protein mixtures (MudPIT)
  • Protein-protein interactions (experimental protocol).
  • Analysis of protein post-translational modifications
  • Protein dynamics by a combination of hydrogen/deuterium and exchange mass spectrometry.
  • Protein quantification by mass spectrometry: iCAT, SILAC, free label, AAUA peptides,...

Last modified: Feb 08, 2017
ID=x11163