Fee Schedule

The facility offers the following services:

  • Gel Imaging
    • Typhoon gel scanner
  • Simple mass measurement
    • Electrospray
    • Sample desalting: Some samples will require desalting; usually this is done using a ZipTip (Millipore) or a C8 RP column 
  • Protein Identification and quantification
    • LC-MS MSMS analysis.

 

Technique

KUMed 

Typhoon Imaging

 

 

Typhon access

10/log

 

One annual Subscription fee

150.00

1D- or 2D- In-gel samples

 

 

Spot picking( manual /robotic)

20 (up to 96)

Proteomics

 

 

Sample clean up

50

 

trypsin digestion (up to 10 samples)

25

Use of other enzymes

cost of enzyme + 50

 

nanoHPLC tandem MSMS analysis (standard run)

125

 

nanoHPLC tandem MSMS analysys (extended run)

250

 

Data analysis-data storage and reporting (per hour)

75

 

Additional chromatographic run (sample previously analyzed)

25

MRM

(costs of reagents not included)

 

Method development

1000

ESI

 

 

 

ESI-low resolution MS  mass measurement

50.00

 

ESI-high resolution MS  mass measurement

100.00

MudPIT

 

 

 

Mud PiT  set up

125

 

each salt step up to fraction # 6

100.00

PTM

 requires HPLC tandem MS chromatographic run

 

 

Additional LC/MS/MS runs (set up conditions, alternative fragmentation methods)

100

 

additional hypothesis driven LC-MS/MS run for PTM's discovery

100

Sequencing

de novo peptde sequecing/Cross-linked peptide (per hour)

75

DHX MS

 

 

 

Feasibility study

1000.00

 

DHX mapping (per condition)

please inquire

Other services

Publication

 preparation of tables and figures for publication

100

Instrument use

 supervised, per day (requires colaboration and training)

75

Computer use

 use of computers in the lab for proteomic applcations

free

Notes:

  1. For most projects the total fee will be the combination of several of these steps. For example, the analysis of a single SDS band will involve, trypsin digestion, HPLC on line tandem MS and 1 h of data analysis (total $250), if effort recovery has been included in a grant, the total will be $175 (until the total number of effort-hours included in the grant have been covered).
  2. We encourage all prospective users of this facility consult with us before submitting any sample for MS analysis.
  3. For grants with effort support, this fee will be subtracted from total, until total effort available in grant is reached, then standard fee will be applied.
  4. There will be an additional 50 % charge for nonprofit organizations not associated with the KU Medical Center, and 100 % for for-profit organizations.
  5. In general, only personnel directly associated with the MSPC have access to the instrumentation. The only exception is for trained personnel from one of the major users groups. In his case, the PI is charged for the initial training as well as the use of the instrument. 
  6. The fee structure can be changed without previous notice.
  7. Special techniques: all other methodologies will be examined on a project per project basis.

Notes to operational procedures:

  1. To prevent cross-contamination, we use a single column per user or sample. What you get is what you have. 

Documents: Colaborative research

Last modified: Feb 08, 2017
ID=x11164