Skip to main content

Pricing

NovaSeq 6000

The following pricing for NovaSeq 6000 sequencing includes initial sample QC*, library preparation, library validation and qPCR quantification at the designated read count or X coverage. Read count and X coverage can be adjusted in 5M read or 5X increments. (*Additional sample QC subject to charge)

The KUMC Bioinformatics Core is available to provide sequence data analysis services. Please contact Sumedha Gunewardena sgunewardena@kumc.edu for service information.

S1 Flow Cell (0.5Tb)    &     S2 Flow Cell (1Tb) Pricing

Service

KUMC / KU, KS Regents, Frontiers InvestigatorsNon-Affiliated Academic Investigators

S1                  S2 

S1                   S2 

NuGEN Universal Plus mRNA-seq w/UDI
25M reads
2x100bp

$335              $310

$420                $390

TruSeq Stranded mRNA
25M reads
2x100bp

$400              $375

$500               $470

TruSeq Stranded Total RNA
40M reads
2x100bp

$550              $510

$690               $640

TruSeq RNA Access
25M reads
2x100bp

$515               $495

$645               $620

TruSeq Small RNA
5M.reads
2x100bp

$315               $310

$395                $390

TruSeq Rapid Exome
25M reads / 100X - (5Gb)
2x100bp

$410                $380

$515                $475

TruSeq ChIP
30M reads
2x100bp

$415               $385

$520                $485

Additional 5M read increments

$29                 $24

$36                  $30

Human Genome
30X coverage
2x150bp

$2365             $2045

$2960              $2560

TruSeq DNA Methylation Sequencing:
Human 30X coverage
2x150bp

$2390             $2070

$2990              $2590

Reduced Representation DNA Methylation Sequencing:
Human 30X coverage (2.2Gb)
2x100bp

$470               $460

$590                 $575

Other Genomes

Inquire

Inquire

Additional X Coverage

Inquire

Inquire

 

S4 Flow Cell (3 Tb) Pricing
ServiceKUMC / KU, KS Regents, Frontiers InvestigatorsNon-Affiliated Academic Investigators

Human Genome
30X coverage
2x150bp

$1405 $1760

TruSeq DNA Methylation Sequencing:
Human 30X coverage
2x150bp

$1435 $1800

Other Genomes

Inquire Inquire

Additional X Coverage

Inquire Inquire

Please direct inquiries to: cbloomer@kumc.edu

HiSeq 2500

High Output (8 lane)
ServiceDescriptionKUMC/KU, KS Regents, Frontiers InvestigatorsNon-Affiliated Academic Investigators
TruSeq v3 Sequencing & Clustering
Cost/lane
100 cycle paired end read  $2275 $2845
50 cycle paired end read $1785 $2235
100 cycle single read $1505 $1885
50 cycle single read $1210 $1515
Dual Index surcharge $100 $125

 

Rapid Read (2 lane)
ServiceDescriptionKUMC/KU, KS Regents, Frontiers InvestigatorsNon-Affiliated Academic Investigators
HiSeq Rapid v2 Sequencing & Clustering
Cost/lane
250 cycle paired end read  $3585 $4485
150 cycle paired end read $2920 $3650
100 cycle paired end read $2365 $2960
50 cycle paired end read $1915 $2395
250 cycle single read $2390 $2990
150 cycle single read $1660 $2075
100 cycle single read $1660 $2075
50 cycle single read $1385 $1735
Dual Index surcharge $100 $125

 

Sequence Library Preparation (see descriptions below)
ServiceDescriptionKUMC/KU, KS Regents, Frontiers InvestigatorsNon-Affiliated Academic Investigators
Cost/sample
Library prep. includes initial sample QC,
library validation,
qPCR quantification.
Additional sample QC
subject to charge.
TruSeq Stranded mRNA $260 $325
TruSeq Stranded Total RNA $330 $415
NuGEN Universal Plus mRNA $195 $245
TruSeq RNA Access $375 $470
TruSeq Small RNA $285 $360
TruSeq DNA PCR Free $230 $290
TruSeq DNA Nano $230 $290
TruSeq ChIP-Seq $250 $315
TruSeq Rapid Capture Exome $260 $325
TruSeq DNA Methylation sequence $255 $320
Reduced Representation Bisulfite Sequence $405 $505
Indexing Fee $10 $15
Library Quant. (qPCR) $20 $25

Library Description

NuGEN Universal Plus mRNA-seq Library w/ UDI – Employs oligo dT enrichment of messenger RNA to assess expression including alternative transcripts and gene fusions. Provides strand of origin to detect antisense transcription. Unique Dual Index (UDI) adapter compatible.

  • Input range of total RNA 10ng – 1ug. Targeted input 500ng.
  • Sequencing recommendation – 25M 100bp paired end reads*.

TruSeq Stranded Total RNA Library – Includes Ribosomal reduction to enrich for mRNA and non-coding transcript interrogation. Provides strand of origin to detect antisense transcription. Compatible with FFPE RNA. Unique Dual Index (UDI) adapter compatible.

  • Input range of total RNA 100ng – 1ug. Targeted input 1ug.
  • Sequencing recommendation – 40M 100bp paired end reads*.

TruSeq mRNA Access Library – Couples TruSeq Stranded mRNA library preparation with sequence-specific probes to capture and enrich mRNA transcripts. Provides strand of origin to detect antisense transcription. Compatible with low yield, low quality and FFPE total RNA. Targeted input variable based on RNA quality.

  • Targeted input of total RNA 10ng. Input range of low quality RNA or FFPE RNA 20ng – 100ng.
  • Sequencing recommendation – 25M 100bp paired end reads*.

TruSeq Small RNA Library – Enables library preparation of any miRNA with a 3’ hydroxyl and a   5’ monophosphate present from Dicer/Drosha processing. Used to find novel miRNAs, characterize variation with single base resolution and analyze differential expression.

  • Targeted input – 1ug total RNA or 10-50 ng miRNA from extracellular vesicles.
  • Sequencing recommendation – 5M reads*.

TruSeq DNA PCR-Free Library – Preferred library preparation for whole genome sequencing when good yields of intact, high molecular weight gDNA are available. Does not incorporated a PCR amplification of the libraries negating any potential for the introduction of amplification bias. Two library sizing options are available: 350bp & 550bp insert. Unique Dual Index (UDI) adapter compatible.

  • Targeted input of high MW gDNA: 350bp library – 1ug, 550bp library – 2ug.
  • Sequencing recommendation – 30 X sequencing coverage*.

TruSeq DNA Nano Library – Preferred library preparation for whole genome sequencing when limited yields of intact, high molecular weight gDNA are available. Two library sizing options are available: 350bp & 550bp insert. Unique Dual Index (UDI) adapter compatible.

  • Targeted input of high MW gDNA: 350bp library – 100ng, 550bp library – 200ng.
  • Sequencing recommendation – 30 X sequencing coverage*.

TruSeq ChIP-Seq Library – Used as an epigenetic analysis tool, chromatin immunoprecipitation (ChIP) sequencing libraries leverage next-generation sequencing (NGS) to determine the distribution and abundance of DNA bound protein targets of interest across the genome. Fragmented control input chromatin and immunoprecipitated chromatin are provided by the investigator. A gel image of the high MW chromatin and fragmented chromatin are required for project submission (fragmented size range 600bp – 100bp).

  • Targeted input of fragmented chromatin: control input - 20ng, immunoprecipitated chromatin - 20ng.
  • Sequencing recommendation:  20M – 40M reads* determined by predicted genome distribution of target protein.

TruSeq Rapid Capture Exome Library – Used for targeted resequencing in human applications. Indexed DNA libraries are prepared for pooled enrichment using exome capture probes targeting 45Mb of coding sequence from >98% of RefSeq, CCDS and Ensemble coding content. Unique Dual Index (UDI) adapter compatible.

  • Targeted input of intact, high MW gDNA – 50ng.
  • Sequencing recommendation – 20M 100bp paired end reads* covering 4Gb.

TruSeq DNA Methylation Library – Produces whole genome bisulfite sequencing libraries from gDNA using a unique “post-bisulfite” library construction method which yields diverse libraries with uniform CpG, CHG and CHH coverage.

  • Targeted input – 50 -100ng RNase treated high MW gDNA.
  • Sequencing recommendation – 30 X sequencing coverage*.

Reduce Representation Bisulfite Sequence Library – The NextFlex Bisulfite-Seq kit uses MspI restriction of genomic DNA to enrich for CpG rich genomic regions reducing the sequencing demand to interrogate methylated gDNA.

  • Targeted input – 2-3ug RNase treated high MW gDNA.
  • Sequencing recommendation – 30 X sequencing coverage* (2.2Gb for human).

*Read counts and X coverage can be modified to meet specific project demands.

Last modified: Jul 26, 2018
ID=x1820