Services Overview

Illumina Services

Sequencing

The Illumina HiSeq 2500 sequencing services provided by the Genome Sequencing Facility (GSF) allows for both the standard High Output 8 lane flow cell run and the 2 lane flow cell Rapid Run. Both services are supported by Paired-End (PE) or Single Read (SR) sequencing using the Illumina TruSeq SBS - HS and TruSeq Rapid SBS - HS chemistry. The High Output runs are available indexed or non-indexed as 50 or 100 cycle PE or SR. The Rapid Run is available indexed or non-indexed as 50, 100 or 150 cycle PE or SR. Flow cell clustering is performed using the cBOT cluster generation station. The following information is a comparison of expected output and data quality for both High Output and Rapid Read runs.

                                                Rapid (2 lane)        High Output (8 lane)    

Cluster Density                      750-900 k/mm²               750-850 k/mm²

Clusters/Lane (PF)                   130 - 150M                 187 - 210M

Flow Cell Output (2x50bp)       25 - 30Gb                       150Gb

Flow Cell Output (2x100bp)    50 - 60Gb                       300Gb

Flow Cell Output (2x150bp)    75 - 90Gb                not supported

Bases >Q30 (2x50bp)                    85%                              85%

Bases >Q30 (2x100bp)                  80%                              80%

Bases >Q30 (2x150bp)                  75%                     not supported

Run times between the Rapid Read and High Output are significantly different. The following provides a comparison of run times.

Run Format               Rapid Read                High Output

 50                               ~ 8 hours                       ~ 2 days

2 x 50                          ~ 16 hours                     ~ 5 days

2 x 100                        ~ 27 hours                     ~ 10 days

2 x 150                        ~ 40 hours                  not supported

Library Preparation

Sequencing library construction is also available through the GSF using the Illumina TruSeq Sample preparation kits. The following library services are offered:

TruSeq mRNA - Generate mRNA libraries directly from total RNA.

TruSeq DNA - Generate Libraries from Genomic DNA.

TruSeq ChIP-Seq DNA - Selectively sequence DNA sequences bound by     target proteins.

TruSeq Small RNA - Generate small RNA libraries directly from total RNA.

TruSeq Exome - Ideal for scalable exome sequencing studies.

For other library constructs please inquire.