Scheduling/Sorting procedures
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Flow Cytometry Core Laboratory Home
Lab: Michael Stewart, MHSA. mstewart2@kumc.edu (913)588-9850
Seminar series:
Department of Microbiology, Molecular Genetics and Immunology
Research at the University of Kansas Medical Center
Advisory Committee
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Useful Links in Flow: BD spectral viewer Invitrogen spectral viewer Molecular Probes Handbook (for all things fluorescent) Handbook Chapter 15 Apoptosis Apoptosis reference Apo-Direct for Apoptosis detection Caspase detection via CaspSCREEN for non-adherent cells Apoptosis Information Autofluorescence, how to avoid or diminish BD Flow Application Notes – useful tips Clontech fluorescent proteins Conjugation of monocolonal antibodies – do it yourself guide Conjugation of antibodies by kit Fluorescent Protein Spectra (see Table 1) General information on sample prep
Links to training tutorials: Introduction to flow Diva software Invitrogen fluorescence tutorials Compensation Interpreting flow cytometry data Statistics for flow cytometry Safety: Biosafety Publications Sorting biosafety introduction Sorting biosafety guidelines CDC guidelines
Other: 17 color Flow Excitation & Emission of living material Non-specific binding
For more info on protocols: Click Here
For info on choosing a Dye: Click Here
To make reservations with The Flow Cytometry Core: Meeting Room Manager