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Flow Cytometry Core Laboratory
School of Medicine  :  Department of Microbiology, Molecular Genetics and Immunology

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The flow core also houses a biosafety cabinet, incubator, and centrifuge so that cells can be cultured and treated within the core.


Instrumentation
These instruments were purchased through a generous endowment from the Hall Foundation with the exception of the Calibur which was donated by the KUMC Dept of Medicine. The day to day operation is maintained by NIH Grant Number P20 RR016443 from the COBRE program of the National Center for Research Resources. 

  • BD LSRII – flow cytometric analysis of single cell/bead suspension
    Lasers:      405nm, 488nm, and 633nm – use dye selection link

  • BD FACS Aria – Fluorescence Activated Cell Sorter© for collecting specified population(s) from single cell/bead suspension.
    Lasers:  405nm, 488nm, and 633nm – use dye selection link

  • BD Calibur – for analysis of single cell/bead suspension
    Lasers:  488nm and 633nmusing dye selection link , select PMTs B, C, & D from 488 list and PMT B from 633 list 
    Detection:  dye selection link below use PMTs B, C, & D from 488 list and PMT B from 633 list

  • CompuCyte iCys – for analysis of adherent cells and tissue sections using fluorescent and chromatice dyes. 
    Objectives:  10, 20, 40, & 60X plus confocol
    Lasers:  405nm, 488nm, and 633nm – dye selection should be discussed with flow core personnel. 
    http://www.compucyte.com/QIC_Teaser/QIC_Teaser.html

  • Luminex 200 – A flexible analyzer based on the principles of flow cytometry that is designed to meet the needs of  research and clinical laboratories. Built on xMAP technology, this system allows multiplexing of up to 100 analytes in a single well of a microtiter plate, using very small sample volumes and is compatible with kits designed for the Luminex 100 instrument. The system delivers fast and cost-effective bioassay results on many assay formats including nucleic acid assays,
    receptor-ligand assays, immunoassays and enzymatic assays.  Kits are available from a number of companies for a wide range of species.
    (http://www.luminexcorp.com/partners/index.html)

  • RoboSep – An automated cell separation instrument that uses bead-based technology to separate cells from single cell suspensions. http://www.stemcell.com/product_catalog/robosep.aspx?

  • Celigo – Scan plates in minutes to collect brightfield images for cell counting and/or fluorescence for the assay you design: apoptosis, proliferation, GFP expression, RFP expression, mitochondrial potential, and many more measurements http://www.cyntellect.com/content/products/celigo/

For more info on protocols: Click Here

For info on choosing a Dye: Click Here

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