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LSR II Dye Selection Guide

Choose only one dye for each PMT.
Please discuss your choices with flow core personel as not all combinations are workable.

Excitation Laser	Detector	Bandpass Filter	Filter nm	Dye
Violet 405 nm	PMT A	740 LP	780/60
	PMT B	670 LP	705/70
	PMT C	630 LP	640/40
	PMT D	595 LP	605/40
	PMT E	505 LP	525/50
	PMT F		450/50

Blue 488 nm	FSC Diode		488/10	Forward Scatter
	PMT A	685 LP	695/40
	PMT B	505 LP	530/10
	PMT C		488/10	Side Scatter


Green 552 nm	PMT A		780/40	PE-Cy7
	PMT B	685 LP	710/50	PE-Cy5.5
	PMT C	635 LP	670/30
	PMT D	600 LP	610/20
	PMT E		575/26

Red 637 nm	PMT A	750 LP	780/60
	PMT B	685 LP	710/50
	PMT C		660/20

Last modified: Feb 18, 2013

Scheduling

No walk-ins with samples, please!

Reservations for use of the analyzers can be made through Meeting Room Manager. Access to meeting room manager is granted upon the successful training of the investigator by the Flow Cytometry Core Director.

Reservations for the FACSAria can be made by e-mailing Flow Cytometry Core Laboratory staff.

Events

Information for:

Academics

Flow Cytometry Core Laboratory

LSR II Dye Selection Guide