Lab:
Alicia Duren:
Flow Cytometry Technician Hemenway 3037
.
aduren@kumc.edu
(913)588-9850
Department of Microbiology, Molecular Genetics and Immunology
Choose only one dye for each PMT.
Please discuss your choices with flow core personelle
as not all combonations are workable.
Please note the following are not options:
BFP, CFP, TEXAS RED, Mrfp, HOESCHT, FURA-2, INDO-1, INDO-RED
,or any of the ALEXA 500s.
| Excitation Laser | Detector | Filter nm | Dye |
| Blue 488nm | FSC Diode | 488/10 (483-493) | Forward Scatter |
| PMT A | 780/60 (750-810) | PE-Cy7 | |
| PMT B | 695/40 (675-715) | ||
| PMT C | 576/26 (563-589) | ||
| PMT D | 530/30 (515-545) | ||
| PMT E | 488/10 (483-493) | Side Scatter | |
| Red 633nm | PMT A | 780/60 (750-810) | |
| PMT B | 710/50 (685-735) |
||
| PMT C | 660/20 (515-545) | ||
| Violet 405nm | PMT A | 530/30 (515-545) | |
| PMT B | 450/40 (430-470) | ||
Click image to enlarge
For more info on protocols: Click Here
To make reservations with The Flow Cytometry Core: Meeting Room Manager
Other helpful information:
Cell Sorting- Basic Idea
Flow Detection- Basic Idea
Flow Detection problems with debris- Basic Idea
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The University of Kansas Medical Center