Flow Cytometry Core Laboratory

The flow cytometry core is located on the third floor of the Kansas Life Science Innovation Center.  The core maintains one each BD LSRII, BD FACS Aria, BD FACS Calibur, and CompuCyte Laser Scanning Cytometer (iCys) within the 550 sq. ft. lab space.  The LSRII, Aria and iCys have three lasers each: 405nm violet, 488nm blue and 633nm DiHeNe; the BD instruments are capable of detecting up to eight fluorometric measurements simultaneously while the iCys can detect four fluorochromes plus two chromatic dyes.  The Calibur has the 488nm and 633nm lasers with the ability to detect four fluorochromes.  Additionally, the core maintains a Luminex 200 specifically designed for the detection of soluble molecules in cell culture media or body fluids, in the picogram range; new applications for this instrument include mapping of signaling pathways via phosphorylation of specific protein targets.  These instruments allow scientist to make very concise measurements at the cellular level and evaluate a large number of samples in a short time frame.

These instruments were purchased through a generous endowment from the Hall Foundation with the exception of the Calibur which was donated by the KUMC Dept of Medicine. The day to day operation is maintained by NIH Grant Number P20 RR016443 from the COBRE program of the National Center for Research Resources.  The core is directed by Joyce Slusser, PhD

• BD LSRII – flow cytometric analysis of single cell/bead suspension Lasers:  405nm, 488nm, and 633nm – use dye selection link
• BD FACS Aria – Fluorescence Activated Cell Sorter© for collecting specified population(s) from single cell/bead suspension.
  Lasers:  405nm, 488nm, and 633nm – use dye selection link
• BD Calibur – for analysis of single cell/bead suspension
  Lasers:  488nm and 633nm 
  Detection:  using dye selection link below PMTs B, C, & D from 488 list and PMT B from 633 list
• CompuCyte iCys – for analysis of adherent cells and tissue sections using fluorescent and chromatice dyes. 
  Objectives:  10, 20, 40, & 60X plus confocol
  Lasers:  405nm, 488nm, and 633nm – dye selection should be discussed with flow core personnel. 
• Luminex 200 – A flexible analyzer based on the principles of flow cytometry that is designed to meet the needs of  research and clinical laboratories. Built on xMAP technology, this system allows multiplexing of up to 100 analytes in a single well of a microtiter plate, using very small sample volumes and is compatible with kits designed for the Luminex 100 instrument. The system delivers fast and cost-effective bioassay results on many assay formats including nucleic acid assays,
  receptor-ligand assays, immunoassays and enzymatic assays.  Kits are available from a number of companies for a wide range of species.
  (http://www.luminexcorp.com/partners/index.html)

No walk-ins with samples, please! If you have specific questions about fluorochromes, dyes or combinations you can use, please call or email. The Aria will run cells at concentrations up to 2X10^7 per ml at about one ml per 30 min. The length of your sort is therefore relative to the abundance the population of interest and your desired # of recovered cells.
Thanks for including flow cytometry in your experiment!

For more info on protocols: Click Here

For info on choosing a Dye: Click Here

To make reservations with The Flow Cytometry Core: Meeting Room Manager