This page is an overview of the basic daily procedures and requirements for the use and handling of biohazardous materials at KUMC. Biohazardous materials include all biological materials in a laboratory or medical setting and present a potential risk to human health. Please refer to the KUMC Biosafety Plan for a more detailed discussion on biosafety.
Some common categories of biohazardous materials are:
Biohazardous materials are common to most labs on the KUMC campus. All researchers should be trained in proper handling techniques, personal decontamination, and basic equipment decontamination appropriate for the materials being used in their labs.
Biosafety cabinets must be certified for use with BSL (Bio-Safety Level) of the materials you are using, and must have up-to-date inspections. Individual laboratories arrange for inspections by contacting the EHS office at firstname.lastname@example.org.
Personal protective garments must be worn properly, and must be suitable to the level of risk.
Decontamination solutions must be easily accessible in all areas in which biohazardous materials are used, and must be suitable to the materials being used in that area.
For information about select toxins, please follow the link below:
The Biohazardous Waste Management Program has been produced in accordance to the EPA, OSHA, and Kansas State regulations. Stericycle is the contractor who handles all of the biohazardous waste that is shipped off campus. All biohazardous waste must be contained within a 28 gallon red tub (supplied by Stericycle). Biohazardous waste must be discarded in red or orange biohazardous waste bags. All biohazardous waste is removed from the labs by the Environment, Health & Safety (EHS) office. If your waste requires autoclaving, ensure that the bag you use is autoclave safe. Please consult the Biological Waste Disposal Instructions for more information about the disposal of biohazardous waste.
Biohazardous waste can be defined as contaminated or potentially contaminated:
Types of biohazardous waste include (but are not limited to):
1. Cultures, stocks of infectious agents and associated biologicals including:
2. Human blood, blood products, body fluids, and cell lines.
3. All sharps (contaminated and uncontaminated) such as:
4. Carcasses, body parts and bedding from animals exposed to pathogens in research. (See Animal facility protocol for waste management)
5. Other laboratory wastes including but not limited to:
Some biohazard waste needs to be rendered "non-viable" before disposal. This includes liquid waste that will be disposed of in the sewer system. Please contact the Environment, Health and Safety (EHS) office prior to disposal of 10 liters or greater. Materials that must be rendered non-viable include any material that has been generated inside a BSL3 laboratory and any materials that contain or have come into contact with:
Acceptable methods of rendering materials "non-viable" include autoclaving or using disinfectants, such as bleach or certain alcohols, known to effectively kill infectious agents. Each of these methods must be done properly in order to be effective. Please consult your supervisor or the EHS office before attempting to disinfect or autoclave materials if you have not been appropriately trained in these methods. Also consult the Selected EPA-registered Disinfectants website for approved disinfectants.
If you are unsure whether you waste needs to be rendered "non-viable", please contact your supervisor or contact the EHS office and we can assist you in making that determination.
There are many autoclaves located on campus. All are supervised by an autoclave monitor and by the EHS office. Biological indicators are run quarterly to test the efficacy of each autoclave. Results are kept in the EHS office if you require them. In the event that an indicator was to fail, the use of the autoclave would be immediately stopped until maintenance was provided and additional biological indicators pass the efficacy test.
After determining whether your waste needs to be autoclaved and can be autoclaved, the proper training and access to the autoclave rooms needs to be obtained. Please contact your supervisor for more information. Certain precautions need to be taken when handling autoclave waste and when transporting waste to the autoclave rooms. Please follow the general guidelines:
Most autoclave rooms are equipped with the 28 gallon red tubs. Place all autoclaved waste into these tubs for removal. If you notice these tubs getting full, please contact the appropriate autoclave monitor.
If you have any questions, please contact the EHS office at 8-1081 or email@example.com.